| Full text | |
| Author(s): |
Roque-Borda, Cesar Augusto
[1]
;
Kulus, Dariusz
[2]
;
Vacaro de Souza, Angela
[3]
;
Kaviani, Behzad
[4]
;
Vicente, Eduardo Festozo
[3]
Total Authors: 5
|
| Affiliation: | [1] Sao Paulo State Univ Unesp, Sch Agr & Veterinarian Sci, BR-14884900 Jaboticabal, SP - Brazil
[2] UTP Univ Sci & Technol Bydgoszcz, Fac Agr & Biotechnol, Lab Ornamental Plants & Vegetable Crops, Bernardynska 6, PL-85029 Bydgoszcz - Poland
[3] Sao Paulo State Univ UNESP, Sch Sci & Engn, BR-17602496 Tupa, SP - Brazil
[4] Islamic Azad Univ, Rasht Branch, Dept Hort Sci, Rasht 4147654919 - Iran
Total Affiliations: 4
|
| Document type: | Review article |
| Source: | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES; v. 22, n. 11 JUN 2021. |
| Web of Science Citations: | 0 |
| Abstract | |
Numerous environmental and endogenous factors affect the level of genetic diversity in natural populations. Genetic variability is the cornerstone of evolution and adaptation of species. However, currently, more and more plant species and local varieties (landraces) are on the brink of extinction due to anthropopression and climate change. Their preservation is imperative for the sake of future breeding programs. Gene banks have been created worldwide to conserve different plant species of cultural and economic importance. Many of them apply cryopreservation, a conservation method in which ultra-low temperatures (-135 degrees C to -196 degrees C) are used for long-term storage of tissue samples, with little risk of variation occurrence. Cells can be successfully cryopreserved in liquid nitrogen (LN) when the adverse effect of ice crystal formation and growth is mitigated by the removal of water and the formation of the so-called biological glass (vitrification). This state can be achieved in several ways. The involvement of key cold-regulated genes and proteins in the acquisition of cold tolerance in plant tissues may additionally improve the survival of LN-stored explants. The present review explains the importance of cryostorage in agronomy and presents an overview of the recent works accomplished with this strategy. The most widely used cryopreservation techniques, classic and modern cryoprotective agents, and some protocols applied in crops are considered to understand which parameters provide the establishment of high quality and broadly applicable cryopreservation. Attention is also focused on the issues of genetic integrity and functional genomics in plant cryobiology. (AU) | |
| FAPESP's process: | 16/00446-7 - Application of antimicrobial peptides as new additives in animal nutrition |
| Grantee: | Eduardo Festozo Vicente |
| Support Opportunities: | Research Grants - Young Investigators Grants |
| FAPESP's process: | 18/25707-3 - Application of the antimicrobial peptide Ctx(Ile21)-Ha and its conjugates in different formulations as a nutritional additive in laying hens |
| Grantee: | Cesar Augusto Roque Borda |
| Support Opportunities: | Scholarships in Brazil - Master |