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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Radiotherapy changes the salivary proteome in head and neck cancer patients: evaluation before, during, and after treatment

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Ventura, Talita Mendes Oliveira [1] ; Ribeiro, Nathalia Regina [1] ; Taira, Even Akemi [1] ; de Lima Leite, Aline [1] ; Dionizio, Aline [1] ; Rubira, Cassia Maria Fischer [2] ; da Silva Santos, Paulo Sergio [2] ; Buzalaf, Marilia Afonso Rabelo [1]
Total Authors: 8
[1] Univ Sao Paulo, Dept Biol Sci, Discipline Biochem, Bauru Sch Dent, Al Octavio Pinheiro Brisolla 9-75, BR-1701290 Bauru, SP - Brazil
[2] Univ Sao Paulo, Dept Surg Stomatol Pathol & Radiol, Discipline Radiol & Stomatol, Bauru Sch Dent, Al Octavio Pinheiro Brisolla 9-75, BR-1701290 Bauru, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Web of Science Citations: 0

Objectives Salivary glands are affected during radiotherapy in the head and neck region, leading to a reduction in salivary flow and changes its composition. Besides negatively affecting the oral soft tissues, this can also lead to dental impairment. Thus, we evaluated the effect of radiotherapy in the proteomic profile of the saliva in patients with head and neck cancer (HNC). Materials and methods HNC patients had their saliva collected before (BRT), during (2-5 weeks; DRT), and after (3-4 months; ART) radiotherapy. Saliva was also collected from healthy volunteers (control; C). Samples were processed for proteomic analysis. Results In total, 1055 proteins were identified, among which 46 were common to all groups, while 86, 86, 286, and 395 were exclusively found in C, BRT, DRT, and ART, respectively. Remarkably, alpha-enolase was increased 35-fold DRT compared with BRT, while proline-rich proteins were decreased. ART there was a 16-fold increase in scaffold attachment factor-B1 and a 3-fold decrease in alpha-enolase and several cystatins. When compared with C, salivary proteins of BRT patients showed increases cystatin-C, lysozyme C, histatin-1, and proline-rich proteins Conclusion/clinical revelance Both HNC and radiotherapy remarkably change the salivary protein composition. Altogether, our results, for the first time, suggest investigating alpha-enolase levels in saliva DRT in future studies as a possible biomarker and strategy to predict the efficiency of the treatment. Moreover, our data provide important insights for designing dental products that are more effective for these patients and contribute to a better understanding of the progressive changes in salivary proteins induced by radiotherapy. (AU)

FAPESP's process: 17/05031-2 - Proteomic analysis of the acquired enamel pellicle and saliva in patients with head and neck cancer that are submitted radiotherapy
Grantee:Talita Mendes Oliveira Ventura
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 18/17860-6 - Proteomic analysis of acquired enamel pellicle in patients with head and neck cancer submitted to radiotherapy
Grantee:Nathalia Regina Ribeiro
Support type: Scholarships in Brazil - Scientific Initiation