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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Soybean extract modified by Aspergillus awamori stimulates a greater collagen-I synthesis in the intracellular matrix of human fibroblasts

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Author(s):
Bezerra, Patricia Heloise Alves [1] ; Stocco, Bianca [1] ; Bianchi, Caroline Inacio [1] ; Bianchini, Francine [1] ; Figueiredo, Sonia Aparecida [2] ; Fonseca, Maria Jose Vieira [2] ; Torqueti, Maria Regina [1]
Total Authors: 7
Affiliation:
[1] Univ Sao Paulo, Sch Pharmaceut Sci Ribeirao Preto, Dept Clin Anal Toxicol & Food Sci, Lab Clin Cytol, Ribeirao Preto, SP - Brazil
[2] Univ Sao Paulo, Dept Pharmaceut Sci, Lab Photochemoprotect, Sch Pharmaceut Sci Ribeirao Preto, Ribeirao Preto, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Journal of Cosmetic Dermatology; JUN 2021.
Web of Science Citations: 0
Abstract

Aglycone isoflavones are estrogen-like bioactive compounds found in low amounts in soybean, which are increased by biotransformation processes. This study investigated two biotransformation processes of soybean extracts with Aspergillus awamori fungus, evaluating aglycone content and capability of stimulation of collagen-I deposition. Isoflavones were quantified via HPLC; cytotoxicity of biotransformed extracts toward mouse and human fibroblasts was evaluated via NRU and apoptosis/necrosis assays; and collagen-I deposition was measured through Western blot, immunofluorescence, and immunoassay. BSE-2 was the biotransformed soybean extract with the highest aglycone content and did not decrease viability or demonstrated cytotoxicity to either L929 or HDFa cells. BSE-2, at the optimal concentration of 1.33 mu g/mL, increased substantially collagen-I amount in HDFa intracellular matrix compared to non-biotransformed soybean extract (NBSE) and immunoassay demonstrated that the extracellular deposition was mostly inhibited by BSE-2 concentrations, except at 1.33 mu g/mL. Hence, biotransformed soybean extract by the enzymatic filtrate of Aspergillus awamori fungus demonstrated a high nutricosmetic potential, showing safeness and effective collagen-I augmentation. (AU)

FAPESP's process: 12/04225-4 - Evaluation of estrogenic potential of soy extract biotransformed in collagen production
Grantee:Maria Regina Torqueti
Support type: Regular Research Grants
FAPESP's process: 12/23271-7 - Evaluation of the safety and efficacy of soy extract biotransformed in cell culture of fibroblasts
Grantee:Caroline Inácio Bianchi
Support type: Scholarships in Brazil - Scientific Initiation