Biodegradation pathway of the organophosphate pesticides chlorpyrifos, methyl parathion and profenofos by the marine-derived fungus Aspergillus sydowii CBMAI 935 and its potential for methylation reactions of phenolic compounds

Author(s)

Soares, Paulo Roberto S. ^[1] ; Birolli, Willian G. ^{[1, 2]} ; Ferreira, Irlon M. ^[3] ; Porto, Andre Luiz M. ^[1]

Affiliation

^[1] Univ Sao Paulo, Sao Carlos Inst Chem, Av Joao Dagnone 1100, Ed Quim Ambiental, BR-13563120 Sao Carlos, SP - Brazil

^[2] Univ Fed Sao Carlos, Ctr Exact Sci & Technol, Chem Dept, Via Washington Luiz, Km 235, BR-13565905 Sao Carlos, SP - Brazil

^[3] Univ Fed Amapa, Campus Ground Zero Ecuador, BR-68902280 Macapa, AP - Brazil

• Document type: Journal article
• Source: Marine Pollution Bulletin; v. 166, MAY 2021.
• Web of Science Citations: 2

Abstract

The indiscriminate use of organophosphate pesticides causes serious environmental and human health problems. This study aims the biodegradation of chlorpyrifos, methyl parathion and profenofos with the proposal of new biodegradation pathways employing marine-derived fungi as biocatalysts. Firstly, a growth screening was carried out with seven fungi strains and Aspergillus sydowii CBMAI 935 was selected. For chlorpyrifos, 32% biodegradation was observed and the metabolites tetraethyl dithiodiphosphate, 3,5,6-trichloropyridin-2-ol, 2,3,5-trichloro-6-methoxypyridine, and 3,5,6-trichloro-1-methylpyridin-2(1H)-one were identified. Whereas 80% methyl parathion was biodegraded with the identification of isoparathion, methyl paraoxon, trimethyl phosphate, O,O, O-trimethyl phosphorothioate, O,O,S-trimethyl phosphorothioate, 1-methoxy-4-nitrobenzene, and 4-nitrophenol. For profenofos, 52% biodegradation was determined and the identified metabolites were 4-bromo-2-chlorophenol, 4-bromo-2-chloro-1-methoxybenzene and O,O-diethyl S-propylphosphorothioate. Moreover, A. sydowii CBMAI 935 methylated different phenolic substrates (phenol, 2-chlorophenol, 6-chloropyridin-3-ol, and pentachlorophenol). Therefore, the knowledge about the fate of these compounds in the sea was expanded, and the marine-derived fungus A. sydowii CBMAI 935 showed potential for biotransformation reactions. (AU)

• FAPESP's process: 14/18257-0 - New approaches in biocatalytic reactions
• Grantee: Andre Luiz Meleiro Porto
• Support Opportunities: Regular Research Grants
• FAPESP's process: 16/20155-7 - Biocatalytic reactions of aminolysis and kinetic resolution by lipases and biodegradation of agrochemicals by microorganisms
• Grantee: Andre Luiz Meleiro Porto
• Support Opportunities: Regular Research Grants
• FAPESP's process: 17/19721-0 - USE OF ENVIRONMENTAL MICRORGANISMS IN THE BIODEGRADATION OF STROBILURINIC AND PYRETHROIDS PESTICIDES WIDELY EMPLOYED IN THE BRAZILIAN AGRICULTURE
• Grantee: Willian Garcia Birolli
• Support Opportunities: Scholarships in Brazil - Post-Doctoral