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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Optimization of (-)-cubebin biotransformation to (-)-hinokinin by the marine fungus Absidia coerulea 3A9

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de Souza, Jonathan Messias [1] ; Conceicao Santos, Mario Ferreira [1] ; Nascimento Pedroso, Rita Cassia [1] ; Pimenta, Leticia Pereira [1] ; Siqueira, Katia Aparecida [2] ; Soares, Marcos Antonio [2] ; Dias, Gustavo Muniz [3] ; Linhari Rodrigue Pietro, Rosemeire Cristina [4] ; Ramos, Henrique Pereira [1] ; Andrade Silva, Marcio Luis [1] ; Pauletti, Patricia Mendonca [1] ; Sola Veneziani, Rodrigo Cassio [1] ; Ambrosio, Sergio Ricardo [1] ; Braun, Glaucia Hollaender [1] ; Januario, Ana Helena [1]
Total Authors: 15
[1] Univ Franca, Ctr Res Exact & Technol Sci, BR-14404600 Franca, SP - Brazil
[2] Univ Fed Mato Grosso, Biosci Inst, Dept Bot & Ecol, BR-78060900 Cuiaba, MT - Brazil
[3] Fed Univ ABC, Ctr Nat & Human Sci, BR-09606070 Sao Bernardo Do Campo, SP - Brazil
[4] Univ Estadual Paulista, Sch Pharmaceut Sci, Dept Drugs & Med, BR-14800903 Araraquara, SP - Brazil
Total Affiliations: 4
Document type: Journal article
Source: Archives of Microbiology; JUN 2021.
Web of Science Citations: 0

The genus Absidia is widely used in the biotransformation of different classes of natural products. This study evaluates the ability of the Absidia coerulea 3A9 marine derived strain isolated from the ascidian Distaplia stilyfera to perform biotransformations by conducting assays with (-)-cubebin, as substrate. The experiment was optimized using the experimental design proposed by Plackett-Burman for seven factors and eight experiments, to establish the biotransformation conditions that would allow maximum production of biotransformed dibenzylbutyrolactone (-)-hinokinin. An analytical method based on Reverse-Phase-High Performance Liquid Chromatography (RP-HPLC) was developed to quantify the fungal biotransformation product. The factor that influenced the (-)-hinokinin peak area the most positively was the percentage of seawater (%seawater) given that its %relative standard deviation (%RSD) showed a 32.92% deviation from the real value. (AU)

FAPESP's process: 07/54762-8 - Obtaining structural analogues of pimaradienoic acid through the process of fungal biotransformation and study of the effect of these metabolites on the activity of the TcDHODH enzyme and on the contraction of the vascular smooth muscle of the aorta artery of mice
Grantee:Sergio Ricardo Ambrosio
Support type: BIOTA-FAPESP Program - Young Investigators Grants
FAPESP's process: 14/19184-7 - Schistosomicidal potential investigation of marine invertebrates belonging to the Ascidiacea family
Grantee:Ana Helena Januário
Support type: Regular Research Grants
FAPESP's process: 17/14261-1 - Investigation of the schistosomicidal, leishmanicidal and cytototoxic potential of fungal lineages associated with marine invertebrates belonging to Ascidiacea
Grantee:Ana Helena Januário
Support type: Regular Research Grants