Angiotensinergic Neurotransmissions in the Medial Amygdala Nucleus Modulate Behavioral Changes in the Forced Swimming Test Evoked by Acute Restraint Stress in Rats

Author(s)

Marchi-Coelho, Camila ^[1] ; Costa-Ferreira, Willian ^{[1, 2]} ; Reis-Silva, Lilian L. ^{[1, 2]} ; Crestani, Carlos C. ^{[1, 2]}

Affiliation

^[1] Sao Paulo State Univ UNESP, Sch Pharmaceut Sci, BR-14800903 Araraquara, SP - Brazil

^[2] Joint UFSCar UNESP Grad Program Physiol Sci, BR-13565905 Sao Carlos, SP - Brazil

• Document type: Journal article
• Source: CELLS; v. 10, n. 5 MAY 2021.
• Web of Science Citations: 0

Abstract

We investigated the role of angiotensin II type 1 (AT(1) receptor) and type 2 (AT(2) receptor) and MAS receptors present in the medial amygdaloid nucleus (MeA) in behavioral changes in the forced swimming test (FST) evoked by acute restraint stress in male rats. For this, rats received bilateral microinjection of either the selective AT(1) receptor antagonist losartan, the selective AT(2) receptor antagonist PD123319, the selective MAS receptor antagonist A-779, or vehicle 10 min before a 60 min restraint session. Then, behavior in the FST was evaluated immediately after the restraint (15 min session) and 24 h later (5 min session). The behavior in the FST of a non-stressed group was also evaluated. We observed that acute restraint stress decreased immobility during both sessions of the FST in animals treated with vehicle in the MeA. The decreased immobility during the first session was inhibited by intra-MeA administration of PD123319, whereas the effect during the second session was not identified in animals treated with A-779 into the MeA. Microinjection of PD123319 into the MeA also affected the pattern of active behaviors (i.e., swimming and climbing) during the second session of the FST. Taken together, these results indicate an involvement of angiotensinergic neurotransmissions within the MeA in behavioral changes in the FST evoked by stress. (AU)

• FAPESP's process: 18/23686-9 - Involvement of angiotensinergic neurotransmissions in the medial nucleus of the amygdala in behavioral changes in the forced swimming test evoked by restraint stress in rats
• Grantee: Camila Marchi Coelho
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 19/24478-3 - Role of the insular cortex in control of the cardiovascular and anxiogenic responses to stress in rats: evaluation of functional topography and involvement of corticotropin-releasing factor (CRF) neurotransmission
• Grantee: Carlos Cesar Crestani
• Support Opportunities: Regular Research Grants
• FAPESP's process: 16/05218-2 - Involvement of the Angiotensin II / AT1 receptor angiotensin 1-7 / Mas receptor signaling in the medial nucleus of the amygdala in the cardiovascular and anxiogenic responses to stress in rats
• Grantee: Willian Costa Ferreira
• Support Opportunities: Scholarships in Brazil - Doctorate