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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Statherin-derived peptides as antifungal strategy against Candida albicans

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Guimaraes Pellissari, Claudia Viviane [1, 2] ; Jorge, Janaina Habib [1] ; Marin, Lina Maria [2] ; Sabino-Silva, Robinson [3] ; Siqueira, Walter Luiz [2]
Total Authors: 5
[1] Sao Paulo State Univ, Araraquara Dent Sch, Dept Dent Mat & Prosthodont, UNESP, 1680 Rua Humaita, BR-14801385 Araraquara, SP - Brazil
[2] Univ Saskatchewan, Coll Dent, 105 Wiggins Rd, Saskatoon, SK S7N 5E4 - Canada
[3] Univ Fed Uberlandia, Inst Biomed Sci, Dept Physiol, 3Q-2121 Av Joao Naves de Avila, BR-38400902 Uberlandia, MG - Brazil
Total Affiliations: 3
Document type: Journal article
Source: ARCHIVES OF ORAL BIOLOGY; v. 125, MAY 2021.
Web of Science Citations: 0

Objective: The aim of this in vitro study was to evaluate the effect of statherin and its naturally occurring peptides (DR9-2, DR9, GE-12, IT-32, GQ-19, IP-18) on Candida albicans metabolism and biofilm development. Design: After the killing assay, a peptide pellicle was formed on the bottom of a polystyrene plate at the IC50 of each peptide. Over the peptide pellicle, Candida albicans biofilm (48 h) was grown. The peptides antimicrobial activity after the peptides treatment was evaluated by alamarBlue, total biofilm biomass and colony forming units (CFU) counting. Results: The pellicle with statherin and the peptides (DR9-2, DR9, GE-12, IP-18, GQ-19) was able to reduce he viability of Candida albicans compared to the negative control. They also decreased cell proliferation by 20 % and total biomass. IT-32 showed the highest reduction in cell proliferation and biomass, which was similar to the positive control, histatin 5. Conclusions: These results suggest that the naturally occuring peptides from statherin are able to decrease Candida albicans colonization and biofilm proliferation. (AU)

FAPESP's process: 16/03847-2 - Antifungal activity and cytotoxicity of biomaterials, in co-culture, coated with chitosan nanoparticles containing encapsulated salivary proteins molecules (histatin 1, histatin 5 or DR9RR14)
Grantee:Janaina Habib Jorge
Support type: Regular Research Grants
FAPESP's process: 15/06016-1 - Antifungal activity and cytotoxicity of biomaterials coated with chitosan nanoparticles containing salivary proteins molecules, histatin 1, histatin 5 or DR9RR14 encapsulated, in co-culture
Grantee:Cláudia Viviane Guimarães Pellissari
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 18/13904-9 - Evaluation of novel salivary peptides for treatment of oral candidiasis
Grantee:Cláudia Viviane Guimarães Pellissari
Support type: Scholarships abroad - Research Internship - Doctorate