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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Expression of virulence factors by Pseudomonas aeruginosa biofilm after bacteriophage infection

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Author(s):
Oliveira, Viviane de Cassia [1, 2] ; Steixner, Stephan [3] ; do Nascimento, Cassio [1] ; Pagnano, Valeria Oliveira [1] ; Silva-Lovato, Claudia Helena [1] ; Oliveira Paranhos, Helena de Freitas [1] ; Wilflingseder, Doris [4] ; Coraca-Huber, Debora [3] ; Watanabe, Evandro [5, 2]
Total Authors: 9
Affiliation:
[1] Univ Sao Paulo, Sch Dent Ribeirao Preto, Dept Dent Mat & Prostheses, Ribeirao Preto, SP - Brazil
[2] Univ Sao Paulo, Sch Nursing Ribeirao Preto, Human Exposome & Infect Dis Network HEID, Ribeirao Preto, SP - Brazil
[3] Med Univ Innsbruck, Dept Orthoped Surg, Res Lab Biofilms & Implant Associated Infect BIOF, Expt Orthoped, Innsbruck - Austria
[4] Med Univ Innsbruck, Div Hyg & Med Microbiol, Innsbruck - Austria
[5] Univ Sao Paulo, Sch Dent Ribeirao Preto, Dept Restorat Dent, Sao Paulo - Brazil
Total Affiliations: 5
Document type: Journal article
Source: Microbial Pathogenesis; v. 154, MAY 2021.
Web of Science Citations: 0
Abstract

The use of bacteriophages for the treatment of bacterial infections has been extensively studied. Nonetheless, the stress response regarding bacteriophage infection and the expression of virulence factors of Pseudomonas aeruginosa after phage infection is poorly discussed. In this study, we evaluated biofilm formation capacity and expression of virulence factors of P. aeruginosa after bacteriophage infection. Biofilm growth rates, biofilm morphology, pyocyanin production and elastase activity were evaluated after 2, 8, 24 and 48 h of co-cultivation with bacteriophages that was recently characterized and showed to be infective towards clinical isolates. In parallel, quantitative real-time polymerase chain reactions were carried out to verify the expression of virulencerelated genes. Bacteriophages promoted substantial changes in P. aeruginosa biofilm growth at early co-culture time. In addition, at 8 h, we observed that some cultures developed filaments. Although bacteriophages did not alter both pyocyanin and protease activity, changes on the expression level of genes related to virulence factors were detected. Usually, lasI, pslA, lasB and phzH genes were upregulated after 2 and 48 h of co-culture. These results highlight the need for extensive investigation of pathways and molecules involved in phage infection, since the transcriptional changes would suggest a response activation by P. aeruginosa. (AU)

FAPESP's process: 20/03405-5 - Potential applicability of bacteriophages on endotracheal tubes: antibacterial and antibiofilm activities
Grantee:Pedro Castania Amadio Domingues
Support Opportunities: Scholarships in Brazil - Technical Training Program - Technical Training
FAPESP's process: 18/09757-0 - Potential applicability of bacteriophages on endotracheal tubes: antibacterial and antibiofilm activities
Grantee:Evandro Watanabe
Support Opportunities: Regular Research Grants
FAPESP's process: 19/13271-9 - Potential applicability of bacteriophages on endotracheal tubes: antibacterial and antibiofilm activities
Grantee:Felipe Lazarini Bim
Support Opportunities: Scholarships in Brazil - Technical Training Program - Technical Training