Angular Leaf Spot Resistance Loci Associated With Different Plant Growth Stages in Common Bean

Author(s):Show less -

Almeida, Caleo Panhoca de ^[1] ; de Carvalho Paulino, Jean Fausto ^[1] ; Bonfante, Gabriel Francesco Janini ^[1] ; Perseguini, Juliana Morini Kupper Cardoso ^[1] ; Santos, Isabella Laporte ^[1] ; Goncalves, Joao Guilherme Ribeiro ^[2] ; Patricio, Flavia Rodrigues Alves ^[3] ; Taniguti, Cristiane Hayumi ^[4] ; Gesteira, Gabriel de Siqueira ^[4] ; Garcia, Antonio Augusto Franco ^[4] ; Song, Qijian ^[5] ; Carbonell, Sergio Augusto Morais ^[2] ; Chiorato, Alisson Fernando ^[2] ; Benchimol-Reis, Luciana Lasry ^[1]

Affiliation

^[1] Inst Agron IAC, Ctr Pesquisa Recursos Genet Vegetais, Campinas - Brazil

^[2] Inst Agron IAC, Ctr Graos & Fibras, Campinas - Brazil

^[3] Inst Biol IB, Lab Fitopatol, Campinas - Brazil

^[4] Univ Sao Paulo, Dept Genet, Escola Super Agr Luiz de Queiroz, Piracicaba - Brazil

^[5] USDA ARS, Soybean Genom & Improvement Lab, Beltsville, MD - USA

• Document type: Journal article
• Source: FRONTIERS IN PLANT SCIENCE; v. 12, APR 13 2021.
• Web of Science Citations: 4

Abstract

Angular leaf spot (ALS) is a disease that causes major yield losses in the common bean crop. Studies based on different isolates and populations have already been carried out to elucidate the genetic mechanisms of resistance to ALS. However, understanding of the interaction of this resistance with the reproductive stages of common bean is lacking. The aim of the present study was to identify ALS resistance loci at different plant growth stages (PGS) by association and linkage mapping approaches. An BC2F3 inter-gene pool cross population (AND 277 x IAC-Milenio - AM population) profiled with 1,091 SNPs from genotyping by sequencing (GBS) was used for linkage mapping, and a carioca diversity panel (CDP) genotyped by 5,398 SNPs from BeadChip assay technology was used for association mapping. Both populations were evaluated for ALS resistance at the V2 and V3 PGSs (controlled conditions) and R8 PGS (field conditions). Different QTL (quantitative trait loci) were detected for the three PGSs and both populations, showing a different quantitative profile of the disease at different plant growth stages. For the three PGS, multiple interval mapping (MIM) identified seven significant QTL, and the Genome-wide association study (GWAS) identified fourteen associate SNPs. Several loci validated regions of previous studies, and Phg-1, Phg-2, Phg-4, and Phg-5, among the 5 loci of greatest effects reported in the literature, were detected in the CDP. The AND 277 cultivar contained both the Phg-1 and the Phg-5 QTL, which is reported for the first time in the descendant cultivar CAL143 as ALS10.1(UC). The novel QTL named ALS11.1(AM) was located at the beginning of chromosome Pv11. Gene annotation revealed several putative resistance genes involved in the ALS response at the three PGSs, and with the markers and loci identified, new specific molecular markers can be developed, representing a powerful tool for common bean crop improvement and for gain in ALS resistance. (AU)

• FAPESP's process: 18/20992-1 - Study of the genetic control of late darkening and tegument color in beans of the carioca type
• Grantee: Cassia Cristina Augusto Pereira
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 18/15526-1 - Genome-wide association study for identifying agronomic candidate loci in common bean
• Grantee: Caléo Panhoca de Almeida
• Support Opportunities: Scholarships abroad - Research Internship - Master's degree
• FAPESP's process: 19/19670-2 - Population genomics and GWAS for disease resistance and identification of Andean pool introduction regions in carioca bean access
• Grantee: Caléo Panhoca de Almeida
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• FAPESP's process: 14/11145-2 - Molecular marker assisted selection using the AB-QTL method and genotyping by sequencing (GBS) toward angular leaf spot and antracnose resistance in common beans
• Grantee: Luciana Lasry Benchimol-Reis
• Support Opportunities: Regular Research Grants
• FAPESP's process: 17/24711-4 - A wide genome association study for resistance to Fusarium oxysporum f. sp. phaseoli and for tegument color in common bean
• Grantee: Luciana Lasry Benchimol-Reis
• Support Opportunities: Regular Research Grants
• FAPESP's process: 17/01753-3 - Marker assisted selection and genotyping by sequenecing (GBS) toward angular leaf resistance in common beans
• Grantee: Caléo Panhoca de Almeida
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 14/08280-5 - Marker assisted selection towards angular leaf spot resistance in common beans
• Grantee: Juliana Morini Küpper Cardoso Perseguini
• Support Opportunities: Scholarships in Brazil - Post-Doctoral