Complete Chromosome-Scale Genome Sequence Resource for Sporisorium panici-leucophaei, the Causal Agent of Sourgrass Smut Disease

Author(s)

Crestana, Gustavo Schiavone ^[1] ; Taniguti, Lucas Mitsuo ^[2] ; dos Santos, Clesivan Pereira ^[1] ; Benevenuto, Juliana ^[1] ; Ceresini, Paulo Cezar ^[3] ; Carvalho, Giselle ^[4] ; Kitajima, Joao Paulo ^[2] ; Monteiro-Vitorello, Claudia Barros ^[1]

Affiliation

^[1] Univ Sao Paulo, Dept Genet, Genom Grp, Luiz de Queiroz Coll Agr, USP ESALQ, BR-13418900 Piracicaba, SP - Brazil

^[2] Mendel Anal Genom SA, BR-04013000 Sao Paulo, SP - Brazil

^[3] Sao Paulo State Univ UNESP, Plant Hlth Rural Engn & Soils Dept, Mol Phytopathol Lab, BR-15385000 Ilha Solteira, SP - Brazil

^[4] SENAI Innovat Inst Biomass, BR-79640250 Tres Lagoas, MS - Brazil

• Document type: Journal article
• Source: MOLECULAR PLANT-MICROBE INTERACTIONS; v. 34, n. 4, p. 448-452, APR 2021.
• Web of Science Citations: 0

Abstract

Here, we present the first complete chromosome-level genome assembly of the smut fungus strain Sporisorium panici-leucophaei SPL10A, the causal agent of the sourgrass (Digitaria insularis) smut disease. Combining Illumina paired-end and Nanopore long reads, we generated a final assembly composed of 23 chromosomes (22 nuclear and one mitochondrial) with 18,915,934 bp. Gene prediction accomplished using extrinsic evidence from the sugarcane smut fungus Sporisorium scitamineum originated a total of 6,402 protein-encoding genes. The secretome (388 proteins) and the effectorome repertoires (68 candidates) were also predicted, given their crucial roles in plant-pathogen interactions. The complete telomere-to-telomere chromosome sequences of this poorly studied fungus will provide a valuable resource for future comparative genomic studies among smuts to unravel their underlying pathogenicity mechanisms. (AU)

• FAPESP's process: 18/24028-5 - Studies on the interaction Sporisorium panici-leucophaei and Digitaria insularis
• Grantee: Gustavo Schiavone Crestana
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 17/13268-2 - Genomic and functional studies of the interaction Sporisorium scitamineum-cana and other smut fungi: advances and challenges
• Grantee: Claudia Barros Monteiro Vitorello
• Support Opportunities: Regular Research Grants
• FAPESP's process: 16/17545-8 - Genes, genomes & transposable elements contribution to plant-microbe interaction: a sugarcane study case
• Grantee: Marie-Anne Van Sluys
• Support Opportunities: Program for Research on Bioenergy (BIOEN) - Thematic Grants