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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Increased expression of interleukin-6 gene in gastritis and gastric cancer

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Author(s):
Santos, M. P. [1] ; Pereira, J. N. [1] ; Delabio, R. W. [1] ; Smith, M. A. C. [2] ; Payao, S. L. M. [1] ; Carneiro, L. C. [3] ; Barbosa, M. S. [3] ; Rasmussen, L. T.
Total Authors: 8
Affiliation:
[1] Fac Med Marilia, Lab Genet, Marilia, SP - Brazil
[2] Univ Fed Sao Paulo, Dept Morfol, Sao Paulo, SP - Brazil
[3] Univ Fed Goias, Inst Patol Trop & Saude Publ, Goiania, Go - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Brazilian Journal of Medical and Biological Research; v. 54, n. 7 2021.
Web of Science Citations: 0
Abstract

Helicobacter pylori (H. pylori) induces an intense inflammatory response, mediated by proinflammatory cytokines, including interleukin (IL)-6 and its membrane receptor (IL-6R), which activates important signaling pathways in the development of gastric disease and cancer. We investigated the gene and protein expression of IL-6 and IL-6R and the influence of polymorphisms rs1800795, rs1800796, and rs1800797 on its gene expression together with H. pylori infection. Furthermore, an in-silico analysis was performed to support our results. Gastric biopsies were obtained from patients with gastric symptoms and patients with gastric cancer (GC) and were divided into groups (Control, Gastritis, and Cancer). H. pylori was detected by PCR. Real-time-qPCR was employed to determine gene expression, and western blot assay was used to analyze protein expression levels. PCR-RFLP was used to characterize IL-6 polymorphisms. Bioinformatics analyses were performed using the Gene Expression Omnibus (GEO) database and GEO2R to screen out differentially expressed genes (DEGs). H. pylori was detected in 43.3% of the samples. Statistically significant differences were found for IL-6 (P=0.0001) and IL-6R (P=0.0005) genes among the three groups, regardless of the presence of H. pylori. Among patients with H. pylori infection, the IL-6 and IL-6R gene and protein expressions were significantly increased, highlighting IL-6 gene overexpression in patients with GC. No statistically significant differences were found for the rs1800795, rs1800796, and rs1800797 polymorphisms compared to IL-6 gene expression. The results indicated that the IL-6 polymorphisms do not influence its expression, but IL-6 and IL-6R expression seems to be altered by the presence of H. pylori. (AU)

FAPESP's process: 16/25562-0 - Gastric cancer molecular and functional approaches in the Brazilian population
Grantee:Marilia de Arruda Cardoso Smith
Support Opportunities: Research Projects - Thematic Grants
FAPESP's process: 18/02008-2 - Analysis of microRNA expression miR-let7a, miR-125a, miR-155 and the genes of interleukin 6 and its receptor-alpha in patients with gastric diseases and in cell culture: a possible association with H. pylori
Grantee:Monica Pezenatto dos Santos
Support Opportunities: Scholarships in Brazil - Master
FAPESP's process: 18/08481-1 - Gene and Protein Interaction among Components of the Inflammatory, Apoptosis and microRNAs Pathways in Gastric Disease and Gastric Cancer
Grantee:Lucas Trevizani Rasmussen
Support Opportunities: Regular Research Grants