Preventive oral supplementation with Bifidobacterium longum 5(1A) alleviates oxazolone-induced allergic contact dermatitis-like skin inflammation in mice

Author(s)

Ribeiro, W. R. ^[1] ; Queiroz, A. G. ^[1] ; Mendes, E. ^[1] ; Casaro, M. B. ^[1] ; Nascimento, C. M. ^[1] ; Coelho, L. S. S. F. ^[1] ; Martins, F. S. ^[2] ; Leite-Silva, V. R. ^{[1, 3]} ; Ferreira, C. M. ^[1]

Affiliation

^[1] Univ Fed Sao Paulo, Inst Environm Chem & Pharmaceut Sci, Dept Pharmaceut Sci, R Sao Nicolau 210, BR-09913030 Diadema, SP - Brazil

^[2] Univ Fed Minas Gerais, Inst Biol Sci, Dept Microbiol, Ave Presidente Antonio Carlos 6627, BR-31970201 Ufmg Belo Horizonte, MG - Brazil

^[3] Univ Queensland, Therapeut Res Ctr, Translat Res Inst, Diamantina Inst, 37 Kent St, Woolloongabba, Qld 4102 - Australia

• Document type: Journal article
• Source: BENEFICIAL MICROBES; v. 12, n. 2, p. 199-209, 2021.
• Web of Science Citations: 1

Abstract

Allergic contact dermatitis (ACD) is a common allergic skin disease that affects individuals subjected to different antigen exposure conditions and significantly impacts the quality of life of those affected. Numerous studies have demonstrated that probiotics suppress inflammation through immunomodulatory effects. In this study, we aimed to evaluate the effect of the probiotic Bifidobacterium longum 5(1A) as a preventive treatment for ACD using an oxazolone-induced murine model. We demonstrated that B. longum 5(1A) exerted a prophylactic effect on oxazoloneinduced ACD-like skin inflammation via reductions in ear and dermal thickness and leucocyte infiltration. The administration of inactivated B. longum 5(1A) did not affect oxazolone-induced ACD-like skin inflammation, suggesting that the bacteria must be alive to be effective. Given that B. longum 5(1A) is an acetate producer, we treated mice with acetate intraperitoneally, which also prevented ear and dermal thickening. Moreover, the tissue levels of the inflammatory cytokines and chemokines interleukin (IL)-10, IL-33, tumour necrosis factor-alpha, chemokine (C-C motif) ligand 2/monocyte chemoattractant protein-1 and chemokine (C-C motif) ligand 5/RANTES were significantly reduced after probiotic treatment, but only IL-33 and IL-10 were reduced when the mice were treated with acetate. These results show that B. longum 5(1A) exerted a potential prophylactic effect on skin inflammation and that acetate represents one potential mechanism. However, other factors are likely involved since these two treatments do not yield the same results. (AU)

• FAPESP's process: 12/50410-8 - Effects of short-chain fatty acids produced by probiotic bacteria in the prophylaxis and treatment of allergic airway inflammation
• Grantee: Caroline Marcantonio Ferreira
• Support Opportunities: Research Grants - Young Investigators Grants
• FAPESP's process: 19/12324-1 - Probiotic as a therapeutic tool in the skin-lung-bowel axis
• Grantee: Caroline Marcantonio Ferreira
• Support Opportunities: Regular Research Grants