Prospecting Biomarkers for Diagnostic and Therapeutic Approaches in Pythiosis

Author(s):Show less -

Chechi, Jessica Luana ^[1] ; Rotchanapreeda, Tiwa ^[2] ; da Paz, Giselle Souza ^[3] ; Prado, Ana Carolina ^[1] ; Oliveira, Alana Lucena ^[3] ; Souza Vieira, Jose Cavalcante ^[1] ; Rabelo Buzalaf, Marilia Afonso ^[4] ; Rodrigues, Anderson Messias ^[5] ; Delazari dos Santos, Lucilene ^{[6, 7]} ; Krajaejun, Theerapong ^[2] ; Gimenes Bosco, Sandra de Moraes ^{[1, 3]}

Affiliation

^[1] Sao Paulo State Univ Unesp, Inst Biosci, Dept Chem & Biol Sci, BR-18618689 Botucatu, SP - Brazil

^[2] Mahidol Univ, Fac Med Ramathibodi Hosp, Dept Pathol, Bangkok 10400 - Thailand

^[3] Sao Paulo State Univ UNESP, Fac Vet Med & Anim Sci FMVZ, BR-18618681 Botucatu, SP - Brazil

^[4] Univ Sao Paulo, Bauru Sch Dent, Dept Biol Sci, BR-17012901 Bauru, SP - Brazil

^[5] Fed Univ Sao Paulo UNIFESP, Dept Microbiol Immunol & Parasitol, Cell Biol Div, BR-04023062 Sao Paulo - Brazil

^[6] Sao Paulo State Univ UNESP, Ctr Study Venoms & Venomous Anim CEVAP, BR-18610307 Botucatu, SP - Brazil

^[7] Sao Paulo State Univ UNESP, Botucatu Med Sch FMB, Grad Program Trop Dis, BR-18618687 Botucatu, SP - Brazil

• Document type: Journal article
• Source: JOURNAL OF FUNGI; v. 7, n. 6 JUN 2021.
• Web of Science Citations: 0

Abstract

Pythiosis, whose etiological agent is the oomycete Pythium insidiosum, is a life-threatening disease that occurs mainly in tropical and subtropical countries, affecting several animal species. It is frequently found in horses in Brazil and humans in Thailand. The disease is difficult to diagnose because the pathogen's hyphae are often misdiagnosed as mucoromycete fungi in histological sections. Additionally, there is no specific antigen to use for rapid diagnosis, the availability of which could improve the prognosis in different animal species. In this scenario, we investigated which P. insidiosum antigens are recognized by circulating antibodies in horses and humans with pythiosis from Brazil and Thailand, respectively, using 2D immunoblotting followed by mass spectrometry for the identification of antigens. We identified 23 protein spots, 14 recognized by pooled serum from horses and humans. Seven antigens were commonly recognized by both species, such as the heat-shock cognate 70 KDa protein, the heat-shock 70 KDa protein, glucan 1,3-beta-glucosidase, fructose-bisphosphate aldolase, serine/threonine-protein phosphatase, aconitate hydratase, and 14-3-3 protein epsilon. These results demonstrate that there are common antigens recognized by the immune responses of horses and humans, and these antigens may be studied as biomarkers for improving diagnosis and treatment. (AU)

• FAPESP's process: 16/10804-8 - Immunoproteomics of the oomycete Pythium insidiosum: antigens candidates for the diagnosis of equine pythiosis
• Grantee: Jéssica Luana Chechi
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 19/08761-7 - Immunoblot of oomycetes Pythium insidiosum: search of candidate antigens for diagnosis of pythiosis
• Grantee: Jéssica Luana Chechi
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• FAPESP's process: 18/08009-0 - New approaches aimed at rapid and differential diagnosis of equine pythiosis
• Grantee: Sandra de Moraes Gimenes Bosco
• Support Opportunities: Regular Research Grants