Advanced search
Start date
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Detection of Dysbiosis and Increased Intestinal Permeability in Brazilian Patients with Relapsing-Remitting Multiple Sclerosis

Full text
Show less -
Pellizoni, Felipe Papa [1] ; Leite, Aline Zazeri [2] ; Rodrigues, Nathalia de Campos [3] ; Ubaiz, Marcelo Jordao [1] ; Gonzaga, Marina Ignacio [1] ; Takaoka, Nauyta Naomi Campos [1] ; Mariano, Vania Sammartino [4] ; Omori, Wellington Pine [5] ; Pinheiro, Daniel Guariz [5] ; Matheucci Junior, Euclides [6] ; Gomes, Eleni [2] ; Oliveira, de Gislane Lelis Vilela [7, 2]
Total Authors: 12
[1] Sch Hlth Sci Dr Paulo Prata, Microbiome Study Grp, BR-14785002 Barretos - Brazil
[2] Sao Paulo State Univ, Inst Biosci Humanities & Exact Sci, Microbiol Program, BR-15054000 Sao Jose Do Rio Preto - Brazil
[3] DNA Consult Genet & Biotechnol, BR-13560340 Sao Carlos - Brazil
[4] Barretos Canc Hosp, BR-14784400 Barretos - Brazil
[5] Sao Paulo State Univ UNESP, Sch Agr & Veterinarian Sci, Dept Technol, BR-14884900 Jaboticabal - Brazil
[6] Univ Fed Sao Carlos, Biotechnol Dept, BR-13565905 Sao Carlos - Brazil
[7] Sao Paulo State Univ UNESP, Food Engn & Technol Dept, BR-15054000 Sao Jose Do Rio Preto - Brazil
Total Affiliations: 7
Document type: Journal article
Web of Science Citations: 0

Dysbiosis, associated with barrier disruption and altered gut-brain communications, has been associated with multiple sclerosis (MS). In this study, we evaluated the gut microbiota in relapsing-remitting patients (RRMS) receiving disease-modifying therapies (DMTs) and correlated these data with diet, cytokines levels, and zonulin concentrations. Stool samples were used for 16S sequencing and real-time PCR. Serum was used for cytokine determination by flow cytometry, and zonulin quantification by ELISA. Pearson's chi-square, Mann-Whitney, and Spearman's correlation were used for statistical analyses. We detected differences in dietary habits, as well as in the gut microbiota in RRMS patients, with predominance of Akkermansia muciniphila and Bacteroides vulgatus and decreased Bifidobacterium. Interleukin-6 concentrations were decreased in treated patients, and we detected an increased intestinal permeability in RRMS patients when compared with controls. We conclude that diet plays an important role in the composition of the gut microbiota, and intestinal dysbiosis, detected in RRMS patients could be involved in increased intestinal permeability and affect the clinical response to DTMs. The future goal is to predict therapeutic responses based on individual microbiome analyses (personalized medicine) and propose dietary interventions and the use of probiotics or other microbiota modulators as adjuvant therapy to enhance the therapeutic efficacy of DMTs. (AU)

FAPESP's process: 17/04508-0 - Metagenomic analysis of fecal microbiota in multiple sclerosis patients and correlation with B and K vitamins' synthesis
Grantee:Aline Zazeri Leite
Support type: Scholarships in Brazil - Scientific Initiation
FAPESP's process: 16/05062-2 - Characterization of gut microbiota isolated from faeces from multiple sclerosis patients and correlation with Th17 cytokines
Grantee:Felipe Papa Pellizoni
Support type: Scholarships in Brazil - Scientific Initiation