| Full text | |
| Author(s): Show less - |
de Almeida, Caleo Panhoca
[1]
;
Santos, Isabella Laporte
[1]
;
de Carvalho Paulino, Jean Fausto
[1]
;
Ferrari Barbosa, Caio Cesar
[1]
;
Augusto Pereira, Cassia Cristina
[1]
;
Limonta Carvalho, Cassia Regina
[1]
;
Cunha Goncalves, Gabriel de Moraes
[2]
;
Song, Qijian
[3]
;
Morais Carbonell, Sergio Augusto
[2]
;
Chiorato, Alisson Fernando
[2]
;
Benchimol-Reis, Luciana Lasry
[1]
Total Authors: 11
|
| Affiliation: | [1] Agron Inst IAC, Nat Ctr Plant Genet, Common Bean Genet Grp, Campinas, SP - Brazil
[2] Agron Inst IAC, Grain & Fiber Ctr, Common Bean Breeding Grp, Campinas, SP - Brazil
[3] USDA ARS, Soybean Genom & Improvement Lab, Beltsville, MD - USA
Total Affiliations: 3
|
| Document type: | Journal article |
| Source: | BMC PLANT BIOLOGY; v. 21, n. 1 JUL 20 2021. |
| Web of Science Citations: | 0 |
| Abstract | |
Background Common bean (Phaseolus vulgaris L.) is a legume whose grain can be stored for months, a common practice among Brazilian growers. Over time, seed coats become darker and harder to cook, traits that are undesirable to consumers, who associate darker-colored beans with greater age. Like commercial pinto and cranberry bean varieties, carioca beans that have darker seeds at harvest time and after storage are subject to decreased market values. Results The goal of our study was to identify the genetic control associated with lightness of seed coat color at harvest (HL) and with tolerance to post-harvest seed coat darkening (PHD) by a genome-wide association study. For that purpose, a carioca diversity panel previously validated for association mapping studies was used with 138 genotypes and 1,516 high-quality SNPs. The panel was evaluated in two environments using a colorimeter and the CIELAB scale. Shelf storage for 30 days had the most expressive results and the L{*} (luminosity) parameter led to the greatest discrimination of genotypes. Three QTL were identified for HL, two on chromosome Pv04 and one on Pv10. Regarding PHD, results showed that genetic control differs for L{*} after 30 days and for the Delta L{*} (final L{*}-initial L{*}); only Delta L{*} was able to properly express the PHD trait. Four phenotypic classes were proposed, and five QTL were identified through six significant SNPs. Conclusions Lightness of seed coat color at harvest showed an oligogenic inheritance corroborated by moderate broad-sense heritability and high genotypic correlation among the experiments. Only three QTL were significant for this trait - two were mapped on Pv04 and one on Pv10. Considering the Delta L, six QTL were mapped on four different chromosomes for PHD. The same HL QTL at the beginning of Pv10 was also associated with Delta L{*} and could be used as a tool in marker-assisted selection. Several candidate genes were identified and may be useful to accelerate the genetic breeding process. (AU) | |
| FAPESP's process: | 18/20992-1 - Study of the genetic control of late darkening and tegument color in beans of the carioca type |
| Grantee: | Cassia Cristina Augusto Pereira |
| Support Opportunities: | Scholarships in Brazil - Scientific Initiation |
| FAPESP's process: | 17/24711-4 - A wide genome association study for resistance to Fusarium oxysporum f. sp. phaseoli and for tegument color in common bean |
| Grantee: | Luciana Lasry Benchimol-Reis |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 19/19670-2 - Population genomics and GWAS for disease resistance and identification of Andean pool introduction regions in carioca bean access |
| Grantee: | Caléo Panhoca de Almeida |
| Support Opportunities: | Scholarships in Brazil - Doctorate (Direct) |