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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

An improved acetic acid-urea polyacrylamide electrophoresis method to evaluate bovine sperm protamines

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Author(s):
dos Santos Hamilton, Thais Rose [1] ; Simoes, Renata [2] ; Ortiz D'Avila Assumpcao, Mayra Elena [1]
Total Authors: 3
Affiliation:
[1] Univ Sao Paulo, Sch Vet Med & Anim Sci, Dept Anim Reprod, Av Prof Orlando Marques de Paiva 87, Cidade Univ, Sao Paulo - Brazil
[2] Fed Univ ABC, Ctr Nat & Human Sci, Santo Andre, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: REPRODUCTION IN DOMESTIC ANIMALS; v. 56, n. 7, p. 1050-1056, JUL 2021.
Web of Science Citations: 0
Abstract

The acetic acid-urea polyacrylamide gel electrophoresis system could separate very similar basic proteins on differences in size and effective charge. This system has been used for many years to analyse histones and their post-translational modifications and widely used in the study of mammal protamines. Two types of protamine have been described, the protamine 1 (P1) and the protamine 2 (P2) family members, which are synthetized by PRM1 and PRM2 genes. The ratio of P1 and P2 is important for predicting fertility in humans and mice. Therefore, the quantification of protamines is a fundamental step in order to establish the ratio between P1 and P2 in these species. In other mammals, studies linking sperm protamination and the protamine ratio with fertility are increasing. So, the use of an effective technique to separate and quantify protamines is important to study sperm P1/P2 ratio. Therefore, this article describes in detail a feasible and useful procedure to isolate bovine sperm protamines, to perform pre-electrophoresis with PEG solution and finally to carry out acid-urea polyacrylamide gel electrophoresis in reverse polarity. This technique allows a clear separation and efficient detection of bovine sperm protamines. (AU)

FAPESP's process: 11/11231-8 - Study of factors involved in DNA fragmentation of spermatozoa in ram.
Grantee:Thais Rose dos Santos Hamilton
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 10/09145-3 - Expression and localization of protamines and transition proteins in bovine sperm
Grantee:Renata Simões
Support Opportunities: Scholarships in Brazil - Post-Doctoral