Analysis of the effects of Photodynamic therapy with Photodithazine on the treatment of 9l/lacZ cells, in vitro study

Author(s)

Vitorio, Gabrielle dos Santos ^[1] ; de Almeida, Rainara Moreno Sanches ^[1] ; Pinto, Juliana Guerra ^[1] ; Fontana, Leticia Correa ^[1] ; Ferreira-Strixino, Juliana ^[1]

Affiliation

^[1] Univ Vale Paraiba, Inst Res & Dev, Lab Photobiol Appl Hlth, Ave Shishima Hifumi 2911, BR-12244000 Sao Jose Dos Campos, SP - Brazil

• Document type: Journal article
• Source: Photodiagnosis and Photodynamic Therapy; v. 34, JUN 2021.
• Web of Science Citations: 0

Abstract

Gliosarcoma is an aggressive brain tumor. Photodynamic Therapy (PDT) is a treatment that can be used for various cancers of the CNS. The aim of this study was to analyze the effects of PDT with Photodithazine (PDZ) in the treatment of gliosarcoma, using 9 L/lacZ cells and serial concentrations of 200 mu g/mL to 3.1 mu g/mL of PDZ. The samples were divided into two groups: dark and light (10 J/cm2). The PDZ was internalized along all the cytoplasmic extension. Viability tests demonstrated a reduction in viable cells after PDT. The production of ROS was concentration-dependent and PDZ was found in mitochondria and lysosomes, presenting a discrete connection with alpha-tubulin. However, this structure is likely damaged, evidenced by changes in the morphological analysis. Thus, according to the parameters of this study, PDZ proved to be an interesting PS in PDT for the treatment of gliosarcoma, with the inherent limitations of an in vitro study. (AU)

• FAPESP's process: 18/12274-1 - Evaluation of photodynamic therapy with Photodithazine in gliossarcoma treatment: in vitro study
• Grantee: Gabrielle dos Santos Vitorio
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 13/07276-1 - CEPOF - Optics and Photonic Research Center
• Grantee: Vanderlei Salvador Bagnato
• Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC
• FAPESP's process: 16/12211-4 - Study of the genotoxic effect, the membrane potential and cell death pathways of curcumin in photodynamic therapy in promastigote and amastigote forms of L. major and L. braziliensis: in vitro
• Grantee: Juliana Ferreira Strixino
• Support Opportunities: Regular Research Grants
• FAPESP's process: 18/15302-6 - Morphological and genotoxic analysis of PDT with fotoenticine in the treatment of gliossarcoma
• Grantee: Rainara Moreno Sanches de Almeida
• Support Opportunities: Scholarships in Brazil - Scientific Initiation