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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Placental Sampling for Understanding Viral Infections - A Simplified Protocol for the COVID-19 Pandemic

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Author(s):
Nobrega, Guilherme de Moraes [1] ; Siqueira Guida, Jose Paulo [1] ; Japecanga, Rodolfo Rosa [1] ; Antolini-Tavares, Arthur [1] ; Mysorekar, Indira [2] ; Costa, Maria Laura [1]
Total Authors: 6
Affiliation:
[1] Univ Estadual Campinas, Rua Alexander Fleming 101, BR-13084881 Campinas, SP - Brazil
[2] Washington Univ, Sch Med, St Louis, MO - USA
Total Affiliations: 2
Document type: Journal article
Source: Revista Brasileira de Ginecologia e Obstetrícia; v. 43, n. 5, p. 377-383, MAY 2021.
Web of Science Citations: 0
Abstract

Abstract Objective The coronavirus disease 2019 (COVID-19) is a pandemic viral disease, caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The impact of the disease among the obstetric population remains unclear, and the study of the placenta can provide valuable information. Adequate sampling of the placental tissue can help characterize the pathways of viral infections. Methods A protocol of placental sampling is proposed, aiming at guaranteeing representativity of the placenta and describing the adequate conservation of samples and their integrity for future analysis. The protocol is presented in its complete and simplified versions, allowing its implementation in different complexity settings. Results Sampling with the minimum possible interval from childbirth is the key for adequate sampling and storage. This protocol has already been implemented during the Zika virus outbreak. Conclusion A protocol for adequate sampling and storage of placental tissue is fundamental for adequate evaluation of viral infections on the placenta. During the COVID-19 pandemic, implementation of this protocol may help to elucidate critical aspects of the SARS-CoV-2 infection. (AU)

FAPESP's process: 19/18720-6 - Characterization of placental Virome and immune transcriptome of pregnant women with Zika Virus infection and high-risk pregnancies
Grantee:Guilherme de Moraes Nobrega
Support Opportunities: Scholarships in Brazil - Scientific Initiation