Optimization of cello-oligosaccharides production by enzymatic hydrolysis of hydrothermally pretreated sugarcane straw using cellulolytic and oxidative enzymes

Author(s)

Barbosa, Fernando Cesar ^[1] ; Kendrick, Emanuele ^[2] ; Brenelli, Livia Beatriz ^{[3, 4]} ; Arruda, Henrique Silvano ^[5] ; Pastore, Glaucia Maria ^[5] ; Rabelo, Sarita Candida ^[6] ; Damasio, Andre ^[7] ; Franco, Telma Teixeira ^{[3, 8]} ; Leak, David ^[2] ; Goldbeck, Rosana ^[1]

Affiliation

^[1] Univ Campinas UNICAMP, Sch Food Engn, Bioproc & Metab Engn Lab, Campinas, SP - Brazil

^[2] Univ Bath, Fac Sci, Dept Biol & Biochem, Bath, Avon - England

^[3] Univ Estadual Campinas, Interdisciplinary Ctr Energy Planning, Campinas, SP - Brazil

^[4] Brazilian Ctr Res Energy & Mat CNPEM, Brazilian Biorenewables Natl Lab LNBR, Campinas, SP - Brazil

^[5] Univ Estadual Campinas, Sch Food Engn, Dept Food Sci, Bioflavors & Bioact Cpds Lab, BR-13083862 Campinas, SP - Brazil

^[6] Sao Paulo State Univ UNESP, Coll Agr Sci, Dept Bioproc & Biotechnol, Botucatu, SP - Brazil

^[7] Univ Campinas UNICAMP, Inst Biol, Dept Biochem & Tissue Biol, BR-13083862 Campinas, SP - Brazil

^[8] Univ Campinas UNICAMP, Fac Chem Engn, Lab Biochem Engn Biorefining & Prod Renewable Ori, Campinas, SP - Brazil

• Document type: Journal article
• Source: BIOMASS & BIOENERGY; v. 141, OCT 2020.
• Web of Science Citations: 7

Abstract

Enzymatic hydrolysis of lignocellulosic biomass accounts for 20-30% of the total cost of second-generation bioethanol production and many efforts have been made in recent years to overcome the high cost of enzymes. Using cello-oligosaccharides (COS), intermediates in cellulose conversion to glucose, may provide advantages over monomeric glucose fermentation, such as lower risk of growth of process contaminants, shorter fermentation time and limited process inhibition by high concentrations of glucose. In addition, COS are also useful as functional oligosaccharides in the food and feed sectors. This study aimed to optimize COS production for further industrial applications. To the best of our knowledge, this is the first study that has used a design of experiments approach to analyze the synergism between endoglucanases, lytic polysaccharide monooxygenase (LPMO), cellobiose dehydmgenase (CDH) and different additives during the hydrolysis of a pretreated sugarcane straw for COS production. After optimization of enzymatic hydrolysis, a combination of the endoglucanases CaCel and CcCel9m, the LPMO TrCe161A, the CDH NcCDHIIa, with lactose and copper as additives, produced 60.49 mg of COS per g of pretreated sugarcane straw, 1.8-2.7-fold more than the commercial enzyme cocktails Cellic (R) Ctec2 and Celluclast (R) 1.5 L. The COS/glucose ratio achieved was 298.31, an increase of 3314 and 2294-fold over the commercial enzymatic cocktails, respectively. These results open a new perspective regarding COS production and its industrial application. (AU)

• FAPESP's process: 17/16159-0 - Optimization of cello-oligosaccharides production by enzymatic hydrolysis of sugarcane straw via heterologous expression of cellulolytic and oxidative enzymes
• Grantee: Fernando César Barbosa
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• FAPESP's process: 17/22669-0 - N-glycosylation and enzymes secretion in filamentous fungi
• Grantee: André Ricardo de Lima Damasio
• Support Opportunities: Program for Research on Bioenergy (BIOEN) - Regular Program Grants
• FAPESP's process: 15/50612-8 - An integrated approach to explore a novel paradigm for biofuel production from lignocellulosic feedstocks
• Grantee: Telma Teixeira Franco
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 19/08542-3 - Enzymatic biotechnology for the xylo-oligosaccharides production from lignocellulosic agroindustrial residues aimed the production of symbiotics
• Grantee: Rosana Goldbeck Coelho
• Support Opportunities: Regular Research Grants