Influence of chitosan-tripolyphosphate nanoparticles on thermosensitive polymeric hydrogels: structural organization, drug release mechanisms and cytotoxicity

Author(s):Show less -

Freitas Mariano, Kelli C. ^[1] ; Monteiro do Nascimento, Monica H. ^[1] ; Querobino, Samyr M. ^[1] ; Ramos Campos, V, Estefania ; de Oliveira, Jhones L. ^[2] ; Yokaichiya, Fabiano ^[3] ; Franco, Margareth K. K. D. ^[4] ; Alberto-Silva, Carlos ^[1] ; de Paula, Eneida ^[5] ; Lombello, Christiane B. ^[6] ; de Lima, Renata ^[7] ; Fraceto, Leonardo F. ^[2] ; de Araujo, Daniele R. ^[1]

Affiliation

^[1] Fed Univ ABC, Human & Nat Sci Ctr, Santo Andre, SP - Brazil

^[2] Ramos Campos, Estefania, V, State Univ Julio de Mesquita Filho, Dept Environm Engn, Sorocaba, SP - Brazil

^[3] Helmholtz Zentrum Berlin Mat, Dept Quantum Phenomena Novel Mat, Berlin - Germany

^[4] Nucl & Energy Res Inst, Sao Paulo, SP - Brazil

^[5] Univ Estadual Campinas, Dept Biochem & Tissue Biol, Inst Biol, Campinas, SP - Brazil

^[6] Fed Univ ABC, Engn Modelling & Appl Social Sci Ctr, Santo Andre, SP - Brazil

^[7] Univ Sorocaba, Dept Biotechnol, Sorocaba - Brazil

• Document type: Journal article
• Source: INTERNATIONAL JOURNAL OF POLYMERIC MATERIALS; v. 69, n. 9, p. 592-603, JUN 12 2020.
• Web of Science Citations: 5

Abstract

Chitosan-tripolyphosphate (CS-TPP) nanoparticles containing naproxen (NPX) were dispersed in poloxamer (PL) as unique (PL407) or binary (PL407-PL403) systems. Nanoparticles presented diameter of similar to 250 nm and zeta potential of similar to 35 mV with drug loading and encapsulation efficiency of 98.4 +/- 0.3% and 36.9 +/- 0.12%, respectively. NPX-CS-TPP shifted the sol-gel transition and micellization temperatures. PL407-PL403 systems presented G ` > G `' compared to PL407. SAXS patterns revealed transitions from lamellar to hexagonal phase organizations with low drug release rates, in the presence of CS-TPP nanoparticles. NPX-CS-TPP-PL407 induced lower cytotoxicity compared to PL407-PL403 in fibroblasts and osteoblasts, making them promising systems for intra-articular delivery. {[}GRAPHICS] . (AU)

• FAPESP's process: 14/14457-5 - Lipid-based nanocarriers (SLN/NLC and remote-loading liposomes) used to improve the upload and potency of local anesthetics
• Grantee: Eneida de Paula
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 14/12653-1 - Development of chitosan-tripolyphosphate nanoparticles dispersed in thermoreversible hydrogels for intra-articular delivery of naproxen
• Grantee: Kelli Cristina Freitas Mariano
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 14/26200-9 - Nanostructured hybrid systems for modified release of antiinflammatpry drugs: development and pharmacological evaluation
• Grantee: Daniele Ribeiro de Araujo
• Support Opportunities: Regular Research Grants