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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Unlikely transmission of Cowpea aphid-borne mosaic virus by hand pollination of passionflower

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Author(s):
Spadotti, David M. A. [1] ; Bibiano, Lillian B. J. [1] ; Rezende, Jorge A. M. [1]
Total Authors: 3
Affiliation:
[1] Univ Sao Paulo, Escola Super Agr Luiz de Queiroz, Dept Fitopatol & Nematol, BR-13418900 Piracicaba - Brazil
Total Affiliations: 1
Document type: Journal article
Source: TROPICAL PLANT PATHOLOGY; v. 46, n. 4, p. 476-480, AUG 2021.
Web of Science Citations: 0
Abstract

Passion fruit plants are self-incompatible and fruiting is exclusively dependent on cross-pollination, which in nature is performed by carpenter (mamangava) bees, especially Xylocopa spp. (family Apidae). To ensure satisfactory productivity, daily manual pollination is a common practice among passionflower growers. Therefore, we studied whether manual pollination mechanically transmits the cowpea aphid-borne mosaic virus (CABMV), the causal agent of the passion fruit woodiness disease. None of the eight healthy passion fruit plants manually pollinated with pollen from CABMV field-infected plants were infected, as confirmed by PTA-ELISA and RT-PCR. Healthy passion fruit plants mechanically inoculated with extracts of pollen collected from flowers of CABMV field-infected plants were not infected. CABMV was only detected by RT-PCR in groups of three anthers, and in pollen from groups of 20 anthers collected from flowers of infected passion fruit plants. RT-qPCR conducted with different amounts of pollen grains and anthers indicated extremely low virus titers. In addition to low virus titers, transmission failure may be associated with an absence of virus particles outside the pollen grains and/or absence of enough wounding produced on the stigmas by the fingertips. The improbability of CABMV transmission via pollen during manual pollination showed that this widely used cultural practice is a negligible factor in the epidemiology of passion fruit woodiness disease. (AU)

FAPESP's process: 16/08573-8 - Cowpea aphid borne mosaic virus (CABMV) control by cultural management and genetic transformation of passionfruit
Grantee:David Marques de Almeida Spadotti
Support Opportunities: Scholarships in Brazil - Post-Doctoral