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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

mall extracellular vesicles derived from in vivo- or in vitro-produced bovine blastocysts have different miRNAs profiles-Implications for embryo-maternal recognitio

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Author(s):
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Bridi, Alessandra [1] ; Andrade, Gabriella M. [1] ; del Collado, Maite [1] ; Sangalli, Juliano R. [1] ; de Avila, Ana C. F. C. M. [1] ; Motta, Igor G. [2] ; da Silva, Julio C. B. [1] ; Pugliesi, Guilherme [2] ; Silva, Luciano A. [1] ; Meirelles, Flavio V. [1] ; da Silveira, Juliano C. [1] ; Perecin, Felipe [1]
Total Authors: 12
Affiliation:
[1] Univ Sao Paulo, Fac Anim Sci & Food Engn, Dept Vet Med, Av Duque Caxias Norte 225, BR-13635900 Pirassununga, SP - Brazil
[2] Univ Sao Paulo, Fac Vet Med & Anim Sci, Dept Anim Reprod, Sao Paulo - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Molecular Reproduction and Development; v. 88, n. 9 AUG 2021.
Web of Science Citations: 1
Abstract

In vivo- and in vitro-produced bovine embryos have different metabolic profiles and differences in gene transcription patterns. These embryos also have a distinct ability to establish and sustain early pregnancies. Small extracellular vesicles (sEVs) are secreted by embryos and carry bioactive molecules, such as miRNAs. We hypothesize that in vivo or in vitro-produced bovine hatched blastocysts on Day 9 and the sEVs secreted by them have different miRNA profiles. To address this hypothesis, embryos of both groups were placed in in vitro culture on Day 7. After 48 h, hatched embryos and hatched embryo-conditioned media (eCM) of both groups were collected. A total of 210 miRNAs were detected in embryos of both groups, of these 6 miRNAs were downregulated, while 7 miRNAs were upregulated in vitro group when compared to in vivo group. sEVs were isolated from eCM to determine miRNA profile. A total of 106 miRNAs were detected in both groups, including 14 miRNAs upregulated in sEVs from in vivo-eCM, and 2 miRNAs upregulated in sEVs from in vitro-eCM. These miRNAs express in embryos and sEVs secreted by them regulate early embryonic developmental and endometrial pathways, which can modify embryo-maternal communication during early pregnancy and consequently affect pregnancy establishment. (AU)

FAPESP's process: 18/13155-6 - Mechanisms of lipid accumulation in oocytes under metabolic altered environments: in vitro maturation and obesity
Grantee:Felipe Perecin
Support Opportunities: Regular Research Grants
FAPESP's process: 15/21829-9 - Multi-user equipment approved in grant 2014/22887-0: Nanosight LM10
Grantee:Juliano Coelho da Silveira
Support Opportunities: Multi-user Equipment Program
FAPESP's process: 17/19681-9 - Extracellular vesicles secreted by in vivo and in vitro produced bovine embryos: miRNA content and molecular effects on endometrium and corpus luteum
Grantee:Alessandra Bridi
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 14/22887-0 - Cell-secreted vesicles containing miRNAs modulate epigenetic changes during in vitro culture of bovine gametes and embryos
Grantee:Juliano Coelho da Silveira
Support Opportunities: Research Grants - Young Investigators Grants