Advanced search
Start date
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Cortisol Dose-Dependently Impairs Migration and Tube-like Formation in a Trophoblast Cell Line and Modulates Inflammatory and Angiogenic Genes

Full text
Rodrigues Hungaro, Talita Guerreiro [1] ; Gregnani, Marcos F. [2] ; Alves-Silva, Thais [2] ; Herse, Florian [3, 4, 5, 6] ; Alenina, Natalia [3, 6, 7] ; Bader, Michael [3, 6, 7, 8, 9] ; Araujo, Ronaldo C. [1, 2]
Total Authors: 7
[1] Fed Univ Sao Paulo UNIFESP, Biophys Dept, Lab Genet & Exercise Metab, Nephrol Program, BR-04039032 Sao Paulo - Brazil
[2] Fed Univ Sao Paulo UNIFESP, Biophys Dept, Lab Genet & Exercise Metab, Mol Biol Program, BR-04039032 Sao Paulo - Brazil
[3] Max Delbruck Ctr Mol Med MDC, Robert Rossle Str 10, D-13125 Berlin - Germany
[4] Expt & Clin Res Ctr ECRC, Lindenberger Weg 80, D-13125 Berlin - Germany
[5] Max Delbruck Ctr Mol Med MDC, Lindenberger Weg 80, D-13125 Berlin - Germany
[6] Berlin Inst Hlth, D-10178 Berlin - Germany
[7] German Ctr Cardiovasc Res DZHK, Partner Site Berlin, D-10117 Berlin - Germany
[8] Charite, Max Delbruck Ctr Mol Med, Charitepl 1, D-10117 Berlin - Germany
[9] Univ Lubeck, Inst Biol, Ratzeburger Allee 160, D-23562 Lubeck - Germany
Total Affiliations: 9
Document type: Journal article
Source: BIOMEDICINES; v. 9, n. 8 AUG 2021.
Web of Science Citations: 0

Several stimuli can change maternal hormone levels during pregnancy. These changes may affect trophoblastic cells and modulate the development of the embryo and the placental tissue itself. Changes in cortisol levels are associated with impaired trophoblast implantation and function, in addition to other pregnancy complications. This study aims to analyze the effects of low and high doses of cortisol on an extravillous trophoblast cell line, and the effects of various exposures to this hormone. SGHPL-4 cells were treated with cortisol at five doses (0-1000 nM) and two exposures (continuous: 24 h/day; and intermittent: 2 h/day). In intermittent treatment, cortisol acted mainly as an anti-inflammatory hormone, repressing gene expression of kinin B1 receptors, interleukin-6, and interleukin-1 beta. Continuous treatment modulated inflammatory and angiogenic pathways, significantly repressing angiogenic factors and their receptors. Cortisol affected cell migration and tube-like structures formation. In conclusion, both continuous and intermittent exposure to cortisol repressed the expression of inflammatory genes, while only continuous exposure repressed the expression of angiogenic genes, suggesting that a sustained increase in the levels of this hormone is more harmful than a high short-term increase. Cortisol also impaired tube-like structures formation, and kinin receptors may be involved in this response. (AU)

FAPESP's process: 19/06981-0 - Role of the B1 kinin receptor in the exercise-induced maternal metabolism during pregnancy and in gestational diabetes: a translational study
Grantee:Ronaldo de Carvalho Araújo
Support Opportunities: Regular Research Grants
FAPESP's process: 15/20082-7 - Kallikrein kinin system in physical exercise and metabolism
Grantee:Ronaldo de Carvalho Araújo
Support Opportunities: Research Projects - Thematic Grants