Lentiviral transduction of neonatal rat ventricular myocytes preserves ultrastructural features of genetically modified cells

Author(s)

Consonni, Silvio R. ^[1] ; de Carvalho, Anna C. P. V. ^[2] ; Mauro, Artur B. ^[2] ; Franchini, Kleber G. ^[2] ; Bajgelman, Marcio C. ^{[3, 4, 2]}

Affiliation

^[1] Univ Campinas UNICAMP, Dept Biochem & Tissue Biol, Campinas, SP - Brazil

^[2] Ctr Res Energy & Mat CNPEM, Brazilian Natl Lab Biosci LNBio, Campinas, SP - Brazil

^[3] Univ Campinas Unicamp, Fac Pharmaceut Sci, Campinas, SP - Brazil

^[4] Univ Campinas UNICAMP, Med Sch, Campinas, SP - Brazil

• Document type: Journal article
• Source: VIROLOGY; v. 562, p. 190-196, OCT 2021.
• Web of Science Citations: 0

Abstract

Preserving morphological features that are important for cell function and structure is a critical parameter for in vitro experiments with rat cardiomyocytes. Lentiviral vectors are commonly used as gene transfer tool because of its high flexibility, efficiency to deliver expression cassettes and versatility of transducing quiescent cells. The tropism of the recombinant viral particle can be determined depending on the virus envelope, which shows a specific binding to cell surface receptors on the target cell. The combination of promoter arrangement and viral envelope must be optimized to achieve a greater transduction efficiency and a higher transgene expression. In this study we explored the optimization of promoters and heterologous envelopes to transduce primary culture of neonatal rat ventricular myocytes. Our results suggest a robust expression driven by the cytomegalovirus promoter, and high efficiency transduction mediated by VSV-G envelope with no apparent compromising ultrastructural features of genetically modified cells. (AU)

• FAPESP's process: 17/21720-2 - Role of Z disc proteins in homeostasis and pathological hypertrophy of cardiac myocytes
• Grantee: Sílvio Roberto Consonni
• Support Opportunities: Regular Research Grants
• FAPESP's process: 19/04458-8 - Development of biological nanoparticles to boost antitumor immunity
• Grantee: Marcio Chaim Bajgelman
• Support Opportunities: Regular Research Grants
• FAPESP's process: 10/17086-7 - Investigation of cellular localization focal adhesion kinase (FAK) and mutant FAK (FAKY397F) in cardiomiocytes in vitro and in genetically modified mice
• Grantee: Sílvio Roberto Consonni
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 12/13132-0 - Exploration of molecular targets to inhibit regulatory T cells and potentiate antitumoral immunity
• Grantee: Marcio Chaim Bajgelman
• Support Opportunities: Regular Research Grants