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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Effect of processing methods on the cytotoxicity of methyl methacrylate-based ocular prostheses: An in vitro study

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Freitas da Silva, Emily Vivianne [1] ; Goiato, Marcelo Coelho [2, 3] ; Bitencourt, Sandro Basso [1] ; Finer, Yoav [4, 5] ; Balera Brito, Victor Gustavo [5] ; Takamiya, Aline Satie [3, 5] ; Penha de Oliveira, Sandra Helena [3, 6] ; Micheline dos Santos, Daniela [2, 3]
Total Authors: 8
[1] Sao Paulo State Univ, Aracatuba Dent Sch, Dept Dent Mat & Prosthodont, UNESP, Jose Bonifacio St 1153, BR-16015050 Aracatuba, SP - Brazil
[2] UNESP, Aracatuba Dent Sch, Dept Dent Mat & Prosthodont, Aracatuba, SP - Brazil
[3] Sao Paulo State Univ, Oral Oncol Ctr, UNESP, Sch Dent, 1193 Jose Bonifacio St, BR-15050015 Aracatuba, SP - Brazil
[4] Univ Toronto, Fac Dent, Toronto, ON - Canada
[5] Univ Toronto, Inst Biomed Engn, Toronto, ON - Canada
[6] UNESP, Dept Basic Sci, Aracatuba Dent Sch, Aracatuba, SP - Brazil
Total Affiliations: 6
Document type: Journal article
Source: TOXICOLOGY IN VITRO; v. 76, OCT 2021.
Web of Science Citations: 0

The study evaluated the influence of cycles and methods of an ocular prosthesis resin on cytotoxicity toward human conjunctival cells. Resins were polymerized by water bath (WB, 74 degrees C or 100 degrees C for 30 min to 9 h), microwave (MW, 1200 W, 3 to 14 min and 30 s at 0 to 720 W), or autopolymerization (AP, room temperature for 20 min +/- 60 degrees C for 30 min). Degree of conversion (DC), cytotoxicity, level of inflammatory mediators, gene expression of different markers, and apoptosis were evaluated. Data were submitted to ANOVA and Tukey test (p < 0.05). WB with longer processing time at higher temperature had highest DC (85.6%) and higher TGF beta 1-gene expression (1.39); long cycle low power MW showed lowest DC (69.6%), lower cell proliferation (85.4%, MTT), and large IL-2 release (39,297 ng/mL). AP with additional processing time showed lower cell proliferation (75.3%, Alamar Blue), and AP polymerized at room temperature showed higher CASP 9-gene expression (1.21). AP methods showed higher IL-6 release (>277 pg/mL). Short cycle medium power MW had higher IL-23 release (534.2 pg/mL). MW (long and short cycles) and AP polymerizations have triggered a more intense inflammatory response. Among methods recommended by the manufacturer, WB showed high DC and less cytotoxicity. (AU)

FAPESP's process: 15/20221-7 - Biocompatibility of ocular prosthesis acrylic resin: in vitro and in vivo analysis
Grantee:Emily Vivianne Freitas da Silva
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 16/03455-7 - Biocompatibility of ocular prosthesis acrylic resin: in vitro and in vivo analysis
Grantee:Daniela Micheline dos Santos
Support type: Regular Research Grants