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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Effect of human dental pulp stem cell conditioned medium in the dentin-pulp complex regeneration: A pilot in vivo study

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Sarra, Giovanna [1] ; de Lima Machado, Manoel Eduardo [1] ; Caballero-Flores, Hector Valentin [1] ; Moreira, Maria Stella [2] ; Fagundes Pedroni, Ana Clara [1, 2] ; Marques, Marcia Martins [2]
Total Authors: 6
[1] Univ Sao Paulo, Sch Dent, Dept Restorat Dent, Sao Paulo, SP - Brazil
[2] Ibirapuera Univ UNIB, Post Grad Program, Sao Paulo, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: TISSUE & CELL; v. 72, OCT 2021.
Web of Science Citations: 0

Background: Dental trauma, restorative operative procedures and/or caries lesions can expose the dental pulp. Facing this clinical condition, where the maintenance of the dentin-pulp complex vitality is imperative, is challenging in Dentistry. Dental pulp stem cells conditioned medium contains trophic factors that could help in this task. This in vivo pilot study aimed to evaluate the effects of the human dental pulp stem cells conditioned medium on the dental pulp tissue response to vital pulp therapy. Material and methods: Concentrated conditioned medium was obtained by incubating characterized human dental pulp stem cells with fresh culture medium. Pulp exposures performed at the first upper molars (n = 20) of Wistar rats were directly capped with: MTA or MTA + Conditioned Medium. Four and 8 weeks later, the samples were qualitatively analyzed in histological sections (H\&E). Results: When the conditioned medium was associated with MTA, there were a high percentage of samples presenting formation of dentin bridges and small percentage of pulp tissue with inflammatory signs in both experimental times. The conditioned medium improved the organization of the newly formed hard tissue. Conclusions: The association of dental pulp stem cell conditioned medium with MTA showed beneficial effects on dentin-pulp complex regeneration and has promising potential for studies in regenerative dentistry. (AU)

FAPESP's process: 17/16777-5 - Investigation of the effect of cell sheet of human dental pulp stem cells associated or not with biophotonics (photobiomodulation therapy and antimicrobial photodynamic therapy) on murine tissue regeneration
Grantee:Marcia Martins Marques
Support Opportunities: Regular Research Grants