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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Biocompatible Graphene Oxide Nanosheets Densely Functionalized with Biologically Active Molecules for Biosensing Applications

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Author(s):
Lehner, Benjamin A. E. [1] ; Benz, Dominik [2] ; Moshkalev, Stanislav A. [3] ; Meyer, Anne S. [4] ; Cotta, Monica A. [5] ; Janissen, Richard [1]
Total Authors: 6
Affiliation:
[1] Delft Univ Technol, Kavli Inst Nanosci, NL-2629 HZ Delft - Netherlands
[2] Delft Univ Technol, Chem Engn, NL-2629 HZ Delft - Netherlands
[3] Univ Estadual Campinas, Ctr Semicond Components & Nanotechnol, BR-13083870 Campinas, SP - Brazil
[4] Univ Rochester, Dept Biol, Rochester, NY 14627 - USA
[5] Univ Estadual Campinas, Dept Appl Phys, Lab Nano & Biosyst, BR-13083859 Campinas, SP - Brazil
Total Affiliations: 5
Document type: Journal article
Source: ACS APPLIED NANO MATERIALS; v. 4, n. 8, p. 8334-8342, AUG 27 2021.
Web of Science Citations: 0
Abstract

Graphene oxide (GO) has immense potential for widespread use in diverse in vitro and in vivo biomedical applications owing to its thermal and chemical resistance, excellent electrical properties and solubility, and high surface-to-volume ratio. However, development of GO-based biological nanocomposites and biosensors has been hampered by its poor intrinsic biocompatibility and difficult covalent biofunctionalization across its lattice. Many studies exploit the strategy of chemically modifying GO by noncovalent and reversible attachment of (bio)molecules or sole covalent biofunctionalization of residual moieties at the lattice edges, resulting in a low coating coverage and a largely bioincompatible composite. Here, we address these problems and present a facile yet powerful method for the covalent biofunctionalization of GO using colamine (CA) and the poly(ethylene glycol) cross-linker that results in a vast improvement in the biomolecular coating density and heterogeneity across the entire GO lattice. We further demonstrate that our biofunctionalized GO with CA as the cross-linker provides superior nonspecific biomolecule adhesion suppression with increased biomarker detection sensitivity in a DNA-biosensing assay compared to the (3-aminopropyl)triethoxysilane cross-linker. Our optimized biofunctionalization method will aid the development of GO-based in situ applications including biosensors, tissue nanocomposites, and drug carriers. (AU)

FAPESP's process: 10/51748-7 - Chemical and structural analysis of Xylella fastidiosa biofilms
Grantee:Mônica Alonso Cotta
Support Opportunities: Regular Research Grants
FAPESP's process: 10/18107-8 - Study of the structures of PANI nanofibers and their hybrids with metallic nanostructures by Raman epectroscopy
Grantee:Marcelo Medre Nobrega
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 13/02300-1 - Semiconductor nanowires: formation mechanisms and biosensing applications
Grantee:Mônica Alonso Cotta
Support Opportunities: Regular Research Grants
FAPESP's process: 15/16611-4 - III-V semiconductor nanowires: synthesis studies for biology applications
Grantee:Mônica Alonso Cotta
Support Opportunities: Regular Research Grants
FAPESP's process: 12/51580-4 - Molecular and functional characterization of proteins potentially related to the phytopatogenicity of biofilm-forming bacteria Xylella fastidiosa
Grantee:Anete Pereira de Souza
Support Opportunities: Regular Research Grants