Nanopore sequencing of tomato mottle leaf distortion virus, a new bipartite begomovirus infecting tomato in Brazil

Author(s)

Martins, Thais P. ^{[1, 2]} ; Souza, Tadeu A. ^[1] ; da Silva, Patricia S. ^[1] ; Nakasu, Erich Y. T. ^[1] ; Melo, Fernando L. ^[2] ; Inoue-Nagata, Alice K. ^[1] ; Nagata, Tatsuya ^[2]

Affiliation

^[1] Embrapa Vegetables, Lab Virol, Brasilia, DF - Brazil

^[2] Univ Brasilia, Dept Mol Biol, Brasilia, DF - Brazil

• Document type: Journal article
• Source: ARCHIVES OF VIROLOGY; v. 166, n. 11 SEP 2021.
• Web of Science Citations: 0

Abstract

During a survey in a tomato field in Luziania (Goias State, Brazil), a single plant with mottling, chlorotic spots, and leaf distortion was found. A new bipartite begomovirus sequence was identified using nanopore sequence technology and confirmed by Sanger sequencing. The highest nucleotide sequence identity match of the DNA-A component (2596 bases) was 81.64% with tomato golden leaf deformation virus (HM357456). Due to the current species demarcation criterion of 91% nucleotide sequence identity for DNA-A, we propose this virus to be a new member of the genus Begomovirus, named ``tomato mottle leaf distortion virus{''}. (AU)

• FAPESP's process: 18/18274-3 - Begomovirus and crinivirus in solanasceous: regional molecular epidemiology and sustainable integrated management alternatives
• Grantee: Jorge Alberto Marques Rezende
• Support Opportunities: Research Projects - Thematic Grants