| Full text | |
| Author(s): |
de Freitas, Emanuelle Neiverth
[1]
;
Alnoch, Robson Carlos
[2]
;
Contato, Alex Graca
[1]
;
Nogueira, V, Karoline Maria
;
Crevelin, Eduardo Jose
[3]
;
Beraldo de Moraes, Luiz Alberto
[3]
;
Silva, Roberto Nascimento
[4]
;
Martinez, Carlos Alberto
[2]
;
Polizeli, Maria de Lourdes T. M.
[2, 4]
Total Authors: 9
|
| Affiliation: | [1] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Bioquim & Imunol, BR-14049900 Ribeirao Preto - Brazil
[2] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Dept Biol, BR-14050901 Ribeirao Preto - Brazil
[3] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Dept Quim, BR-14050901 Ribeirao Preto - Brazil
[4] Nogueira, Karoline Maria, V, Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Bioquim & Imunol, BR-14049900 Ribeirao Preto - Brazil
Total Affiliations: 4
|
| Document type: | Journal article |
| Source: | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES; v. 22, n. 17 SEP 2021. |
| Web of Science Citations: | 0 |
| Abstract | |
Since laccase acts specifically in lignin, the major contributor to biomass recalcitrance, this biocatalyst represents an important alternative to the pretreatment of lignocellulosic biomass. Therefore, this study investigates the laccase pretreatment and climate change effects on the hydrolytic performance of Panicum maximum. Through a Trop-T-FACE system, P. maximum grew under current (Control (C)) and future climate conditions: elevated temperature (2 degrees C more than the ambient canopy temperature) combined with elevated atmospheric CO2 concentration(600 mu mol mol(-1)), name as eT+eC. Pretreatment using a laccase-rich crude extract from Lentinus sajor caju was optimized through statistical strategies, resulting in an increase in the sugar yield of P. maximum biomass (up to 57%) comparing to non-treated biomass and enabling hydrolysis at higher solid loading, achieving up to 26 g L-1. These increments are related to lignin removal (up to 46%) and lignin hydrophilization catalyzed by laccase. Results from SEM, CLSM, FTIR, and GC-MS supported the laccase-catalyzed lignin removal. Moreover, laccase mitigates climate effects, and no significant differences in hydrolytic potential were found between C and eT+eC groups. This study shows that crude laccase pretreatment is a potential and sustainable method for biorefinery solutions and helped establish P. maximum as a promising energy crop. (AU) | |
| FAPESP's process: | 18/07522-6 - Development of eco-friendly technologies for the hydrolysis of biomass and residues of cellulose pulp and paper industry |
| Grantee: | Maria de Lourdes Teixeira de Moraes Polizeli |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 19/07141-5 - Formulation of an optimized synergistic enzyme cocktail with accessory enzymes to enhances the hydrolytic performance of Panicum maximum Jacq. |
| Grantee: | Emanuelle Neiverth de Freitas |
| Support Opportunities: | Scholarships abroad - Research Internship - Doctorate |
| FAPESP's process: | 08/57908-6 - National Institute of Science and Technology of Bioethanol |
| Grantee: | Marcos Silveira Buckeridge |
| Support Opportunities: | Program for Research on Bioenergy (BIOEN) - Thematic Grants |
| FAPESP's process: | 17/23989-9 - Lignocellulosic biomass of Panicum maximum Jacq. forage grass produced in simulated future climate conditions: potential source of fermentable sugars for bioenergy |
| Grantee: | Emanuelle Neiverth de Freitas |
| Support Opportunities: | Scholarships in Brazil - Doctorate |
| FAPESP's process: | 08/58075-8 - Miniface climate-change impact experiment to analyze the effects of elevated CO2 and warming on photosynthesis, gene expression, biochemistry, growth, nutrient dynamics and yield of two contrasting tropical forage species |
| Grantee: | Carlos Alberto Martinez y Huaman |
| Support Opportunities: | Research Program on Global Climate Change - Thematic Grants |
| FAPESP's process: | 20/00081-4 - Development of strategies for immobilization and co-immobilization of enzymes involved in biomass degradation |
| Grantee: | Robson Carlos Alnoch |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |