Evidence of altitudinal gradient modifying genomic and chemical diversity in populations of Lychnophora pinaster Mart.

Author(s):Show less -

Portella, Roberto O. ^{[1, 2]} ; Cordeiro, Erick M. G. ^[3] ; Marques, Ana Paula S. ^[2] ; Ming, Lin C. ^[4] ; Zucchi, I, Maria ; Lima, Maria P. ^[5] ; Martins, Ernane R. ^[6] ; Hantao, Leandro W. ^[7] ; Sawaya, Alexandra C. H. F. ^[8] ; Semir, Joao ^[9] ; Pinheiro, Jose B. ^[10] ; Marques, Marcia O. M. ^[11]

Affiliation:Show less -

^[1] Univ Taubate, Av Tiradentes 500, BR-12030180 Taubate, SP - Brazil

^[2] Univ Estadual Paulista, Inst Biociencias, Dept Bot, Rua Prof Dr Antonio Celso Wagner Zanin 250 Dist R, BR-18618689 Botucatu, SP - Brazil

^[3] I, Agencia Paulista Tecnol Agronegocios, Polo Reg Desenvolvimento Tecnol Ctr Sul, Caixa Postal 28, BR-13400970 Piracicaba, SP - Brazil

^[4] Univ Estadual Paulista, Fac Ciencias Agron, Dept Hort, Rua Jose Barbosa de Barros 1780, BR-18610307 Botucatu, SP - Brazil

^[5] Inst Nacl de Pesquisas da Amazonia, Coordenacao Inovacao Tecnol, Ave Andre Araujo 2936, BR-69011970 Manaus, Amazonas - Brazil

^[6] Univ Fed Minas Gerais, Inst Ciencias Agr, Av Univ 1000, BR-39404547 Montes Claros, MG - Brazil

^[7] Univ Estadual Campinas, Inst Quim, Rua Monteiro Lobato 270, BR-13083862 Campinas, SP - Brazil

^[8] Univ Estadual Campinas, Fac Ciencias Farmaceut, Rua Candido Portinari 200, BR-13083871 Campinas, SP - Brazil

^[9] Univ Estadual Campinas, Inst Biol, Dept Bot, Rua Monteiro Lobato 255, BR-13083862 Campinas, SP - Brazil

^[10] Univ Sao Paulo, Dept Genet, Escola Super Agr Luiz de Queiroz, BR-13418900 Piracicaba, SP - Brazil

^[11] Inst Agron Estado Sao Paulo, Ctr Pesquisa Recursos Genet Vegetais, Ave Barao Itapura 1481, BR-13020902 Campinas, SP - Brazil

• Document type: Journal article
• Source: Phytochemistry; v. 192, DEC 2021.
• Web of Science Citations: 0

Abstract

Lychnophora pinaster Mart. (Asteraceae) is endemic to the Brazilian Cerrado. It is distributed along the altitudinal gradient of the mountainous ranges of the state of Minas Gerais. This study aimed to evaluate the influence of altitude on the genetic diversity of L. pinaster populations and the effects of altitude and climatic factors on essential oil chemical composition. Essential oils from L. pinaster populations from the north (North 01, North 02, and North 03, 700-859 m) and the Metropolitan region of Belo Horizonte (MhBH 01 and MrBH 02, 1366-1498 m) were analyzed. SNP markers from L. pinaster in these regions and Campos das Vertentes (CV 01, CV 02, and CV 03, 1055-1292 m) were also analyzed. The main compounds in essential oils were 14-hydroxy-alpha-humulene (North 01 and North 03), cedr-8(15)-en-9-alpha-ol (North 02), 14-acetoxy-alpha-humulene (MrBH 01), and 4-oxo-15-noreudesman-11-ene (MrBH 02). Hierarchical cluster and heatmap analyses showed that the North and MrBH populations included five different groups, indicating the chemical composition of essential oils is distinct in each population. Furthermore, principal component analysis showed that higher altitudes (1366 m and 1498 m) in the MrBH influence the chemical composition of essential oils, and climatic factors determine the chemical composition in North region. The genetic diversity showed that most alleles are in Hardy-Weinberg equilibrium and imply high genetic variation and genetic polymorphisms between populations. Furthermore, the results of Mantel tests (R = 0.3861517; p = 0.04709529; R = 0.9423121; p = 0.02739726) also showed that higher altitude (>1360 m) shapes the genetic diversity at the MrBH. The genetic structure showed that higher altitudes (>1360 m) contribute to the structure of the MrBH populations, but not to North and CV populations. Therefore, the altitudinal ranges of Minas Gerais mountainous ranges determine the higher genetic and chemical diversity of L. pinaster populations. (AU)

• FAPESP's process: 17/24927-7 - Diversity chemical and genomic of Lychnophora pinaster MART., an important medicinal specie of Brazilian Savannah
• Grantee: Marcia Ortiz Mayo Marques
• Support Opportunities: Regular Research Grants
• FAPESP's process: 20/01064-6 - Analytical developments to study real samples from oil spill in Pernambuco coast: simulations, chemical characterization and ecotoxicity bioassays
• Grantee: Leandro Wang Hantao
• Support Opportunities: Regular Research Grants