Effect of Resistance Training With Total and Partial Blood Flow Restriction on Biomarkers of Oxidative Stress and Apoptosis in Untrained Men

Author(s)

de Lima, Fabio Rocha ^[1] ; Marin, Douglas Popp ^[1] ; Ferreira, Leticia Torres ^[1] ; Sousa Filho, Celso Pereira Batista ^[1] ; Astorino, Todd Anthony ^[2] ; Prestes, Jonato ^[3] ; Marquezi, Marcelo Luis ^[4] ; Otton, Rosemari ^[1]

Affiliation

^[1] Cruzeiro do Sul Univ, Interdisciplinary Postgrad Programme Hlth Sci, Sao Paulo - Brazil

^[2] Calif State Univ San Marcos, Dept Kinesiol, San Marcos, CA - USA

^[3] Univ Catolica Brasilia, Grad Program Phys Educ, Brasilia, DF - Brazil

^[4] Univ Cidade Sao Paulo, Phys Educ Res Lab, Sao Paulo - Brazil

• Document type: Journal article
• Source: FRONTIERS IN PHYSIOLOGY; v. 12, SEP 9 2021.
• Web of Science Citations: 0

Abstract

Introduction: The characterization of immune and oxidative stress responses to acute and chronic exercise training is important because it may aid in the safety and dose-response prescription of resistance training (RT) in many populations.</p> Purpose: The present study compared changes in acute oxidative stress and markers of apoptosis in immune cells before and after 8 weeks of low-load RT with total or partial blood flow restriction (BFR) versus high-load traditional RT.</p> Methods: Twenty-seven untrained men were randomly divided into three groups: traditional RT {[}75% one-repetition maximum (1-RM)], RT with partial (20% 1-RM), and total BFR (20% 1-RM). Over an 8-week period, participants performed six sets of arm curls until failure with 90 seconds of recovery for 3 days/week. Blood samples were obtained before and after the first and last training sessions.</p> Results: Data indicated that all training groups showed similar increases in muscular strength (p < 0.001), reduction in mitochondrial membrane potential (MMP) after exercise in neutrophils (p < 0.001), and increase in caspase-3 activity after exercise (p < 0.001). Traditional RT and total BFR showed increased plasma lipid peroxidation (p < 0.001) and protein carbonyls (p < 0.001) and lower levels of reduced glutathione (GSH) (p < 0.001) after exercise. No change was observed in oxidative stress biomarkers in response to partial BFR (p > 0.05).</p> Conclusion: Data show that RT with partial BFR can increase muscular strength but still does not augment biomarkers of oxidative stress in untrained men. In addition, RT with total BFR promoted similar responses of oxidative stress and markers of immune cell apoptosis versus traditional RT.</p> (AU)

• FAPESP's process: 13/22293-0 - Identification and validation of miRNA target genes in adipose tissue and liver of obese mice in response to treatment with green tea extract
• Grantee: Rosemari Otton
• Support Opportunities: Regular Research Grants