Advanced search
Start date
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Antibody Profile Comparison against MSP1 Antigens of Multiple Plasmodium Species in Human Serum Samples from Two Different Brazilian Populations Using a Multiplex Serological Assay

Full text
Monteiro, Eliana Ferreira [1] ; Fernandez-Becerra, Carmen [2, 3] ; Curado, Izilda [1, 4] ; Wunderlich, Gerhard [5] ; Hiyane, Meire Ioshie [6] ; Kirchgatter, Karin [1, 4]
Total Authors: 6
[1] Univ Sao Paulo, Fac Med, Inst Med Trop, BR-05403000 Sao Paulo - Brazil
[2] Univ Barcelona, ISGlobal, Hosp Clin, Barcelona 08036 - Spain
[3] Germans Trias & Pujol Hlth Sci Res Inst IGTP, Badalona 08916 - Spain
[4] Superintendencia Controle Endemias Estado Sao Paul, Dept Labs Especializados, BR-01027000 Sao Paulo - Brazil
[5] Univ Sao Paulo, Inst Ciencias Biomed, Dept Parasitol, BR-05508000 Sao Paulo - Brazil
[6] Univ Sao Paulo, Inst Ciencias Biomed, Dept Imunol, BR-05508900 Sao Paulo - Brazil
Total Affiliations: 6
Document type: Journal article
Source: PATHOGENS; v. 10, n. 9 SEP 2021.
Web of Science Citations: 0

Plasmodium malariae has a wide geographic distribution, but mainly at very low parasitemias and in co-infections, leading to an underestimated prevalence of this species. Studies for the detection of antibodies against Plasmodium recombinant proteins are increasingly used to map geographical distributions, seroprevalence and transmission intensities of malaria infection. However, no seroepidemiological survey using recombinant P. malariae proteins has been conducted in Brazil. This work evaluated the antibody response in serum samples of individuals from endemic regions of Brazil (the Amazon region and Atlantic Forest) against five recombinant proteins of P. malariae merozoite surface protein 1 (MSP1), and the MSP1 C-terminal portions of P. vivax and P. falciparum, in a multiplex assay. The positivity was 69.5% of samples recognizing at least one MSP1 recombinant protein. The mean of the Reactivity Index for the C-terminal portion of the P. falciparum was significantly higher compared to the other recombinant proteins, followed by the C-terminal of P. vivax and the N-terminal of P. malariae. Among the recombinant P. malariae proteins, the N-terminal of P. malariae showed the highest Reactivity Index alone. This study validates the use of the multiplex assay to measure naturally acquired IgG antibodies against Plasmodium MSP1 proteins and demonstrate that these proteins are important tools for seroepidemiological surveys and could be used in malaria surveillance. (AU)

FAPESP's process: 16/04559-0 - Analysis of phylogeographic and immunological relationship in Plasmodium malariae and Plasmodium brasilianum
Grantee:Karin Kirchgatter Hildebrand
Support Opportunities: Regular Research Grants