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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Copper-Binding Peptides Attenuate Microglia Inflammation through Suppression of NF-kB Pathway

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Author(s):
Caetano-Silva, Maria Elisa [1, 2] ; Rund, Laurie A. [1] ; Vailati-Riboni, Mario [1] ; Bertoldo Pacheco, Maria Teresa [2] ; Johnson, Rodney W. [1]
Total Authors: 5
Affiliation:
[1] Univ Illinois, Dept Anim Sci, Urbana, IL 61801 - USA
[2] Inst Food Technol Ital, Ctr Food Sci & Qual CCQA, BR-13070178 Campinas, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: MOLECULAR NUTRITION & FOOD RESEARCH; v. 65, n. 22 SEP 2021.
Web of Science Citations: 0
Abstract

Scope Activation of microglia, the resident immune cells of the central nervous system, has been related to the etiology and progression of neurodegenerative diseases; thus, finding novel approaches to suppress the neuroinflammatory process is of utmost relevance. Methods and Results The anti-inflammatory activity of whey Cu-, Fe-, and Zn-binding peptides and their possible underlying mechanism of action were evaluated in microglia. Whey metal-binding peptides decreased nitric oxide production and tumor necrosis factor alpha (TNF-alpha) at mRNA and protein levels by stimulated BV-2 microglia in comparison to the control with no peptide treatment. The hydrophobicity, specific sequences, and possible synergistic effects seem to play a role. Cu-binding peptides (Cu-bp) presented anti-inflammatory activity both in BV-2 and primary microglia cultures. These peptides exert their action by suppressing nuclear factor kappa B (NF-kB) pathway since nuclear translocation of NF-kB p65 is decreased by roughly 30% upon Cu-bp treatment. Specific sequences identified in Cu-bp showed high affinity to bind NF-kB p65 by molecular docking (up to -8.8 kcal mol(-1)), corroborating the immunofluorescence studies. Conclusion Cu-bp represent food-derived peptides that may be useful for neuroprotective purposes. Chelation of copper excess in the CNS and the bioavailability of such peptides, as well as their behavior in in vivo models, deserve further research for future applications. (AU)

FAPESP's process: 19/17269-9 - Study of whey peptides activity in the inhibition of microglia activation in cell culture model
Grantee:Maria Elisa Caetano Silva Abreu
Support Opportunities: Scholarships abroad - Research Internship - Post-doctor