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(Reference retrieved automatically from SciELO through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Titanium dioxide nanotubes added to glass ionomer cements affect S. mutans viability and mechanisms of virulence

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Author(s):
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Isaac Jordão de Souza ARAÚJO [1] ; Mariana Gallante RICARDO [2] ; Orisson Ponce GOMES [3] ; Priscila Alves GIOVANI [4] ; Júlia PUPPIN-RONTANI [5] ; Vanessa Arias PECORARI [6] ; Elizabeth Ferreira MARTINEZ [7] ; Marcelo Henrique NAPIMOGA [8] ; Francisco Humberto NOCITI JUNIOR [9] ; Regina Maria PUPPIN-RONTANI [10] ; Paulo Noronha LISBOA-FILHO [11] ; Kamila Rosamilia KANTOVITZ [12]
Total Authors: 12
Affiliation:
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[1] Universidade Estadual de Campinas. Piracicaba Dental School. Department of Restorative Dentistry - Brasil
[2] Faculdade São Leopoldo Mandic. Dental Research Center - Brasil
[3] Universidade Estadual Paulista Júlio Mesquita Filho. School of Sciences. Department of Physics - Brasil
[4] Universidade Estadual de Campinas. Piracicaba Dental School. Department of Pediatric Dentistry - Brasil
[5] Universidade Estadual de Campinas. Piracicaba Dental School. Department of Restorative Dentistry - Brasil
[6] Universidade Paulista. School of Dentistry. Department of Bioestatistics - Brasil
[7] Faculdade São Leopoldo Mandic. Dental Research Center - Brasil
[8] Faculdade São Leopoldo Mandic. Dental Research Center - Brasil
[9] Universidade Estadual de Campinas. Piracicaba Dental School. Department of Prosthodontics and Periodontology - Brasil
[10] Universidade Estadual de Campinas. Piracicaba Dental School. Department of Pediatric Dentistry - Brasil
[11] Universidade Estadual Paulista Júlio Mesquita Filho. School of Sciences. Department of Physics - Brasil
[12] Faculdade São Leopoldo Mandic. Dental Research Center - Brasil
Total Affiliations: 12
Document type: Journal article
Source: Brazilian Oral Research; v. 35, 2021-06-14.
Abstract

Abstract This in vitro study evaluated the impact of TiO2 nanotubes (n-TiO2) incorporated into glass ionomer cement (GIC) on Streptococcus mutans (S. mutans) characteristics at cellular and molecular levels. n-TiO2, synthesized by the alkaline method (20 nm in size), was added to Ketac Molar EasyMix® at 0%, 3%, 5%, and 7% by weight. S. mutans strains were cultured on GIC disks with addition or not of n-TiO2 for 1, 3, and 7 days and the following parameters were assessed: inhibition halo (mm) (n=3/group); cell viability (live/dead) (n=5/group); cell morphology (SEM) (n=3/group); and gene expression by real-time PCR (vicR, covR, gtfB, gtfC, and gtfD) (n=6/group). The data were analyzed by the Kruskal-Wallis test, repeated-measures ANOVA or two-way ANOVA, and Tukey’s and Dunn’s post-hoc tests (α=0.05). The agar diffusion test showed a higher antibacterial property for 5% n-TiO2 compared with 3% and 7% (p<0.05) with no effect of time (1, 3, and 7 days). The cell number was significantly affected by all n-TiO2 groups, while viability was mostly affected by 3% and 5% n-TiO2, which also affected cell morphology and organization. Real-time PCR demonstrated that n-TiO2 reduced the expression of covR when compared with GIC with no n-TiO2 (p<0.05), with no effect of time, except for 3% n-TiO2 on vicR expression. Within-group and between-group analyses revealed n-TiO2 did not affect mRNA levels of gtfB, gtfC, and gtfD (p>0.05). Incorporation of n-TiO2 at 3% and 5% potentially affected S. mutans viability and the expression of key genes for bacterial survival and growth, improving the anticariogenic properties of GIC. (AU)

FAPESP's process: 19/14078-8 - Effect of titanium dioxide nanotubes on biological properties of conventional glass ionomer cement
Grantee:Kamila Rosamilia Kantovitz
Support Opportunities: Regular Research Grants
FAPESP's process: 16/13786-0 - Effect of Novel Nanotechnology to Conventional Glass Ionomer Cement on its Physical-Chemical-Biological Properties
Grantee:Kamila Rosamilia Kantovitz
Support Opportunities: Regular Research Grants