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(Reference retrieved automatically from SciELO through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

5'-Ribonucleotides production using 5'-phosphodiesterase from spent malt roots

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Author(s):
Edson Marcelino Alves [1] ; Joyce Faria de Souza [2] ; Szymon Macieja [3] ; Pedro de Oliva Neto [4]
Total Authors: 4
Affiliation:
[1] Universidade Estadual Paulista Júlio de Mesquita Filho. Faculdade de Ciências e Letras de Assis. Departamento de Biotecnologia - Brasil
[2] Universidade Estadual Paulista Júlio de Mesquita Filho. Faculdade de Ciências e Letras de Assis. Departamento de Biotecnologia - Brasil
[3] Universidade Estadual Paulista Júlio de Mesquita Filho. Faculdade de Ciências e Letras de Assis. Departamento de Biotecnologia - Brasil
[4] Universidade Estadual Paulista Júlio de Mesquita Filho. Faculdade de Ciências e Letras de Assis. Departamento de Biotecnologia - Brasil
Total Affiliations: 4
Document type: Journal article
Source: Brazilian Journal of Food Technology; v. 24, 2021-04-26.
Abstract

Abstract 5'-ribonucleotides are high value-added molecules widely used in the food and pharmaceutical industries because of their bioactive properties. The present work aims to produce a composition of 5’-ribonucleotides using spent brewer’s yeast as cheap source of RNA, and barley malt rootlets as cheap source of 5'-phosphodiesterase (5'-PDE). This is a very promising and innovative strategy because both spent yeast and malt rootles are residues of the brewing process and are closely linked in a cycle that until now is not yet commercially exploited due to lack of studies. Our results showed that extraction of 5’-PDE was mainly influenced by the fineness of the rootlets and amount of extraction solvent (water). The main molecules formed during RNA hydrolysis were 5’-ribonucleotides, which represented 85.86% of the total hydrolyzed molecules. Finally, the results of the proposed approach can generate a new perspective for the brewing industry regarding the management of its wastes, generating from them products of high added value and with a wide range of applications. (AU)

FAPESP's process: 16/08886-6 - Integrated fractionation processes of Saccharomyces cerevisiae from the brewing industry for the production of high value-added biomolecules
Grantee:Edson Marcelino Alves
Support Opportunities: Scholarships in Brazil - Doctorate (Direct)