Load-matched acute and chronic exercise induce changes in mitochondrial biogenesis and metabolic markers

Author(s)

Rodrigues, Natalia Almeida ^[1] ; Gobatto, Claudio Alexandre ^[1] ; Maia Forte, Lucas Dantas ^[1] ; de Barros Sousa, Filipe Antonio ^[2] ; Torsoni, Adriana Souza ^[3] ; de Fante, Thais ^[3] ; Manchado-Gobatto, Fulvia Barros ^[1]

Affiliation

^[1] Univ Estadual Campinas, Sch Appl Sci, Lab Appl Sports Physiol, Limeira, SP - Brazil

^[2] Univ Fed Alagoas, Phys Educ & Sport Inst, Maceio, Alagoas - Brazil

^[3] Univ Estadual Campinas, Sch Appl Sci, Lab Metab Disorders, Limeira, SP - Brazil

• Document type: Journal article
• Source: APPLIED PHYSIOLOGY NUTRITION AND METABOLISM; v. 46, n. 10, p. 1196-1206, OCT 2021.
• Web of Science Citations: 0

Abstract

We investigated the effects of acute and chronic exercise, prescribed in different intensity zones, but with total load-matched on mitochondrial markers (cytochrome C oxidase subunit IV (COX-IV), mitochondrial transcription factor A (Tfam), and citrate synthase (CS) activity in skeletal muscles, heart, and liver), glycogen stores, aerobic capacity, and anaerobic index in swimming rats. For this, 2 experimental designs were performed (acute and chronic efforts). Load-matched exercises were prescribed below, above, and on the anaerobic threshold (AnT), determined by the lactate minimum test. In chronic programs, 2 training prescription strategies were assessed (monotonous and linear periodized model). Results show changes in glycogen stores but no modification in the COX-IV and Tfam contents after acute exercises. In the chronic protocols, COX-IV and Tfam proteins and CS adaptations were intensity- and tissue-dependent. Monotonous training promoted better adaptations than the periodized model. Training at 80% of the AnT improved both performance variables, emphasizing the anaerobic index, concomitant to CS and COX-IV improvement (soleus muscle). The aerobic capacity and CS activity (gastrocnemius) were increased after 120% AnT training. In conclusion, acute exercise protocol did not promote responses in mitochondrial target proteins. An intensity and tissue dependence were reported in the chronic protocols, highlighting training at 80 and 120% of the AnT. Novelty: Load-matched acute exercise did not enhance COX-IV and Tfam contents in skeletal muscles, heart, and liver. In chronic exercise, COX-IV, Tfam, and CS activity adaptations were intensity- and tissue-dependent. Monotonous training was more efficient than the periodized linear model in adaptations of target proteins and enzymatic activity. (AU)

• FAPESP's process: 14/10336-9 - Effect of different training models with load control over physiological parameters, genic expressions and protein contents of HIF-1 alpha, PGC-1 alpha, MCT1 and MCT4: relationship with performance and spontaneous activity of swimming rats
• Grantee: Fúlvia de Barros Manchado Gobatto
• Support Opportunities: Regular Research Grants
• FAPESP's process: 15/00272-6 - Effects of caloric restriction mediated by SIRT3 in the aerobic and anaerobic capacities of mice: relations with the spontaneous activity, metabolic parameters and muscle mitochondrial function in aging process
• Grantee: Fúlvia de Barros Manchado Gobatto
• Support Opportunities: Scholarships abroad - Research
• FAPESP's process: 15/01362-9 - Effects of age, caloric restriction diet (Cr) and SIRT3 on spontaneous activity, behavior and oxidative mediators (NO, NOS and orexin) and hypothalamic mitochondrial respiration
• Grantee: Claudio Alexandre Gobatto
• Support Opportunities: Scholarships abroad - Research