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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Modified ELISA for antibody avidity evaluation: The need for standardization

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Author(s):
Correa, Victor Araujo [1, 2] ; Rodrigues, Thais Sousa [1] ; Portilho, Amanda Izeli [1, 2] ; Lima, Gabriela Trzewikoswki de [1, 2] ; De Gaspari, Elizabeth [1, 2]
Total Authors: 5
Affiliation:
[1] Adolfo Lutz Inst, Immunol Div, Dr Arnaldo Av 355, 11 Floor, Room 1116, BR-01246902 Sao Paulo, SP - Brazil
[2] Univ Sao Paulo, Biomed Sci Inst, Postgrad Program Interunity Biotechnol, Sao Paulo - Brazil
Total Affiliations: 2
Document type: Review article
Source: BIOMEDICAL JOURNAL; v. 44, n. 4, p. 433-438, AUG 2021.
Web of Science Citations: 1
Abstract

Antibody avidity is an important parameter to evaluate immune response, being useful to evaluate vaccine responses and helping to distinguish acute and latent infection. The antibody avidity can be measured by different methods, yet the most common is a modified ELISA. The utilization of commercial kits or in-house methods to evaluate antibody avidity have been adopted more and more, although the lack of standardization between different assays may generate a lot of variation in the process, making it hard to compare the results generated. (AU)

FAPESP's process: 18/17945-1 - Comparative study of ELISA and ELISPOT techniques in the detection of antibodies IgG, IgM in isogenic strains of elderly mice
Grantee:Thais Sousa Rodrigues
Support Opportunities: Scholarships in Brazil - Scientific Initiation
FAPESP's process: 19/02042-9 - Evaluation of response profiles of Th1 and Th2 in mice immunized with anionic and cationic lipids complexed the outer membrane vesicles of Neisseria meningitis b
Grantee:Victor Araujo Correa
Support Opportunities: Scholarships in Brazil - Master
FAPESP's process: 13/11147-2 - Study of the immunogenicity of outer membrane antigens of Neisseria meningitidis and interaction with cationic lipids: evaluation of different immunization schedules in different strains of mice
Grantee:Luciana Tendolini Brito
Support Opportunities: Scholarships in Brazil - Master
FAPESP's process: 18/04202-0 - Study of the immunogenicity of outer membrane antigens of Neisseria meningitidis and interaction with cationic lipids: evaluation of new adjuvant for Neisseria meningitidis in different strains of elderly mice
Grantee:Elizabeth Natal de Gaspari
Support Opportunities: Regular Research Grants
FAPESP's process: 12/15568-0 - Study of the immunogenicity of outer membrane antigens of Neisseria meningitidis and interaction with cationic lipids: evaluation of different immunization schedules in different strains of mice
Grantee:Elizabeth Natal de Gaspari
Support Opportunities: Regular Research Grants