| Full text | |
| Author(s): Show less - |
Munhoz, Jaqueline
[1]
;
Peron, Gabriela
[2]
;
Bonfanti, Amanda Pires
[1, 2]
;
Oliveira, Janine
[2]
;
da Rocha-e-Silva, Thomaz A. A.
[3]
;
Sutti, Rafael
[4]
;
Thome, Rodolfo
[2, 5]
;
Bombeiro, Andre Luis
[2]
;
Barreto, Natalia
[1, 2]
;
Chalbatani, Ghanbar Mahmoodi
[6]
;
Gharagouzloo, Elahe
[7]
;
Vitorino-Araujo, Joao Luiz
[8]
;
Verinaud, Liana
[2]
;
Raposo, Catarina
[1]
Total Authors: 14
|
| Affiliation: | [1] Univ Estadual Campinas UNICAMP, Fac Ciencias Farmaceut, Candido Portinari 200, Cidade Univ, BR-13083871 Campinas, SP - Brazil
[2] Univ Estadual Campinas, Inst Biol, Dept Biol Estrutural & Func, Av Bertrand Russel, BR-13083865 Campinas, SP - Brazil
[3] Fac Israelita Ciencias Saude Albert Einstein, BR-05652900 Sao Paulo, SP - Brazil
[4] Santa Casa Sao Paulo Dr Cesario Motta Jr, Fac Ciencias Med, 61 Vila Buarque, BR-01221020 Sao Paulo, SP - Brazil
[5] Thomas Jefferson Univ, Dept Neurol, 909 Walnut St 3, Philadelphia, PA 19107 - USA
[6] TUMS Sch Med, Dept Immunol & Biol, Poursina Rd, Tehran 1417613151 - Iran
[7] Univ Tehran Med Sci, Canc Inst, Keshavarz Blvd, Tehran 1419733141 - Iran
[8] Fac Ciencias Med Santa Casa Sao Paulo Dona Veridi, Disciplina Neurocirurgia, 56 Higienopolis, BR-01238010 Sao Paulo, SP - Brazil
Total Affiliations: 8
|
| Document type: | Journal article |
| Source: | JOURNAL OF BIOCHEMISTRY; v. 170, n. 1, p. 51-68, JUL 2021. |
| Web of Science Citations: | 1 |
| Abstract | |
Immunomodulation has been considered an important approach in the treatment of malignant tumours. However, the modulation of innate immune cells remains an underexplored tool. Studies from our group demonstrated that the Phoneutria nigriventer spider venom (PnV) administration increased the infiltration of macrophage in glioblastoma, in addition to decreasing the tumour size in a preclinical model. The hypothesis that PnV would be modulating the innate immune system led us to the main objective of the present study: to elucidate the effects of PnV and its purified fractions on cultured macrophages. Results showed that PnV and the three fractions activated macrophages differentiated from bone marrow precursors. Further purification generated 23 subfractions named low weight (LW-1 to LW-12) and high weight (HW-1 to HW-11). LW-9 presented the best immunomodulatory effect. Treated cells were more phagocytic, migrated more, showed an activated morphological profile and induced an increased cytotoxic effect of macrophages on tumour cells. However, while M1-controls (LPS) increased IL-10, TNF-alpha and IL-6 release, PnV, fractions and subfractions did not alter any cytokine, with the exception of LW-9 that stimulated IL-10 production. These findings suggest that molecules present in LW-9 have the potential to be used as immunoadjuvants in the treatment of cancer. (AU) | |
| FAPESP's process: | 15/04194-0 - Identification of new molecules with chemotherapeutic effect in human glioma and characterization of the mechanism |
| Grantee: | Catarina Raposo Dias Carneiro |
| Support type: | Research Grants - Young Investigators Grants |
| FAPESP's process: | 15/06134-4 - Multi-User Equipment approved in grant 2014/03002-7: cell imaging multi-mode reader |
| Grantee: | Marcelo Bispo de Jesus |
| Support type: | Multi-user Equipment Program |
| FAPESP's process: | 18/23559-7 - Cell therapy with macrophages and NK cells modulated ex vivo by peptide isolated from animal venom: a new approach in immunotherapy for cancer |
| Grantee: | Amanda Pires Bonfanti |
| Support type: | Scholarships in Brazil - Doctorate |
| FAPESP's process: | 18/03051-9 - Identification of macrophages modulators molecules from spider venom: new perspectives for the treatment of cancer |
| Grantee: | Jaqueline de Lima Munhoz |
| Support type: | Scholarships in Brazil - Scientific Initiation |
| FAPESP's process: | 19/10003-3 - Molecular characterization of human gliomas and identification of neoplasms responsible for new biopharmaceuticals obtained from animal venom: a translational approach |
| Grantee: | Natália Barreto dos Santos |
| Support type: | Scholarships in Brazil - Doctorate |