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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Antenatal corticosteroid therapy modulates hepatic AMPK phosphorylation and maternal lipid metabolism in early lactating rats

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Author(s):
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Hecht, Fernanda Ballerini [1] ; Teixeira, Caio Jordao [2] ; de Souza, Dailson Nogueira [1] ; Nunes Mesquita, Filiphe de Paula [1] ; do Val Roso, Ryana Elyzabeth [3] ; Sodre, Frhancielly Shirley [2] ; Veronesi, Vanessa Barbosa [1] ; da Rocha, Deborah Fabiana [3] ; Dantas de Menezes, Yan Guida [3] ; Pioli, Mariana Rodrigues [1] ; Bordin, Silvana [2] ; Cecatti, Jose Guilherme [3] ; Luz, Adriana Gomes [3] ; Anhe, Gabriel Forato [1]
Total Authors: 14
Affiliation:
[1] Univ Estadual Campinas, Sch Med Sci, Dept Translat Med, Campinas, SP - Brazil
[2] Univ Sao Paulo, Inst Biomed Sci, Dept Physiol & Biophys, Sao Paulo - Brazil
[3] Univ Estadual Campinas, Sch Med Sci, Dept Obstet & Gynecol, Campinas - Brazil
Total Affiliations: 3
Document type: Journal article
Source: BIOMEDICINE & PHARMACOTHERAPY; v. 144, DEC 2021.
Web of Science Citations: 0
Abstract

Antenatal corticosteroid therapy is used to reduce neonatal mortality in preterm infants but it is currently unknown whether this intervention affects lipid metabolism at the peripartum. This study aimed to evaluate if antenatal corticosteroid therapy in pregnant rats and women affects lipid metabolism during early lactation. We evaluated women at risk of preterm delivery that received corticosteroid therapy (CASE) and women that were not exposed to corticosteroid and were not at risk of preterm delivery (CONTROL). Samples were collected to measure serum and milk triacylglycerol (TAG) three days after delivery. Rats were treated with dexamethasone (DEX) between the 15th and the 20th days of pregnancy. Samples were collected at different days after delivery (L3, L8 and L14). TAG was measured in serum, liver and mammary gland (MG). TAG appearance rates were measured after tyloxapol injection and gavage with olive oil. We also evaluated the expression of key genes related to lipid metabolism in the liver and in the MG and hepatic phosphorylation of AMP-activated protein kinase (AMPK) and acetyl-CoA carboxylase (ACC). CASE volunteers delivered earlier than CONTROL but presented unaltered milk and serum TAG concentrations. Early lactating DEX rats exhibited increased TAG in serum, MG and milk. No changes in CD36 and LPL were detected in the MG and liver. Early lactating DEX rats displayed increased TAG appearance rate and reduced hepatic AMPK/ACC phosphorylation. Our data revealed that antenatal corticosteroid therapy reduces hepatic AMPK/ACC phosphorylation during early lactation that reflects in increased TAG concentration in serum, MG and milk. (AU)

FAPESP's process: 19/03196-0 - Molecular mechanisms involved in the metabolic inflexibility of rats submitted to metabolic programming induced by prenatal excess of glucocorticoids
Grantee:Silvana Auxiliadora Bordin da Silva
Support type: Regular Research Grants
FAPESP's process: 13/07607-8 - OCRC - Obesity and Comorbidities Research Center
Grantee:Licio Augusto Velloso
Support type: Research Grants - Research, Innovation and Dissemination Centers - RIDC
FAPESP's process: 20/09717-9 - Molecular mechanisms involved in cardiovascular changes triggered by fetal excess glucocorticoids
Grantee:Frhancielly Shirley Souza Sodré
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 16/13138-9 - Effect of PPAR-gamma or PPAR-alpha agonists on the metabolic programing induced by maternal caloric restriction
Grantee:Vanessa Barbosa Veronesi
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 20/06397-3 - Study of cellular senescence in rodents subjected to Obesity
Grantee:Caio Jordão Teixeira
Support type: Scholarships in Brazil - Post-Doctorate