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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Chitosan/tripolyphosphate nanoformulation carrying paraquat: insights on its enhanced herbicidal activity

Full text
Author(s):
Pontes, Montcharles S. [1] ; Antunes, Debora R. [2] ; Oliveira, Ivan P. [3] ; Forini, Mariana M. L. [2] ; Santos, Jaqueline S. [1] ; Arruda, Gilberto J. [1] ; Caires, Anderson R. L. [4, 5] ; Santiago, Etenaldo F. [1] ; Grillo, Renato [2]
Total Authors: 9
Affiliation:
[1] Mato Grosso do Sul State Univ UEMS, Ctr Nat Resources Study CERNA, Nat Resources Program, Plant Resources Study Grp, Dourados, MS - Brazil
[2] Sao Paulo State Univ UNESP, Fac Engn, Dept Phys & Chem, Ilha Solteira, SP - Brazil
[3] Univ Sao Paulo, Inst Biomed Sci, Dept Pharmacol, Sao Paulo, SP - Brazil
[4] Univ Essex, Sch Life Sci, Colchester, Essex - England
[5] Fed Univ Mato Grosso do Sul UFMS, Inst Phys, Opt & Photon Grp, Campo Grande, MS - Brazil
Total Affiliations: 5
Document type: Journal article
Source: ENVIRONMENTAL SCIENCE-NANO; v. 8, n. 5, p. 1336-1351, MAY 1 2021.
Web of Science Citations: 1
Abstract

Despite the wide range of possible applications of nanopesticides, the mechanisms involved in their enhanced action remain largely unknown. Understanding the interaction between nanopesticides and plants is crucial for evaluating their potential safety application. Using an experimental and theoretical approach, this study aimed to investigate the target effect of paraquat-loaded chitosan/tripolyphosphate nanoparticles (ca. 200 nm) on photosystem I (PSI). Electrochemical analysis showed distinct electroactivity behaviour between the encapsulated and non-encapsulated herbicide. The amount of lipid peroxidation, photooxidizable P700 reaction centre content, and NADPH/NADP(+) ratio levels were significantly decreased in spinach leaf tissue exposed to the nanoherbicide compared to those with the non-encapsulated herbicide. The data also revealed that the nanoformulation might promote oxidative stress based on changes observed in antioxidant enzymes. Also, molecular docking results showed a preferential disposition of the herbicide paraquat and paraquat-tripolyphosphate complex (TPP:PQ) in the ligand domain close to FAD and Glu312. Due to the inhibitor's strategic position in the catalytic pocket, a model of electron-capture is proposed, where the herbicide disturbs the redox process NADP(+) NADPH by capturing electrons to reduce itself. Finally, our findings provide important insights where the redox behaviour of paraquat may play a key role in the enhanced efficiency of nanoherbicides to the target binding site. (AU)

FAPESP's process: 17/02201-4 - Study of the inhibition of the accumulation of cyclic nucleotide by pyridopyrimidine derivatives using molecular dynamics simulations
Grantee:Ivan Pires de Oliveira
Support Opportunities: Scholarships in Brazil - Post-Doctoral
FAPESP's process: 20/12769-0 - Gold nanoparticles encapsulated in nanocarriers of pesticide as a potential label for understanding its fate in plants
Grantee:Mariana Monteiro de Lima Honorato Forini
Support Opportunities: Scholarships in Brazil - Scientific Initiation
FAPESP's process: 17/21004-5 - Agriculture, micro/nanotechnology and environment: from evaluation of the mechanisms of action to studies of transport and toxicity
Grantee:Leonardo Fernandes Fraceto
Support Opportunities: Research Projects - Thematic Grants
FAPESP's process: 11/01872-6 - Polymeric nanoparticles as delivery system for herbicides: preparation, characterization, interaction with humic substances and assessment of biological activity
Grantee:Renato Grillo
Support Opportunities: Scholarships in Brazil - Doctorate