limate change affects cell-wall structure and hydrolytic performance of a perennial grass as an energy cro

Author(s)

de Freitas, Emanuelle N. ^{[1, 2]} ; Khatri, Vinay ^[2] ; Contin, Daniele R. ^[3] ; de Oliveira, Tassio B. ^[3] ; Contato, Alex G. ^[1] ; Peralta, Rosane M. ^[4] ; dos Santos, Wanderley D. ^[4] ; Martinez, Carlos A. ^[3] ; Saddler, Jack N. ^[2] ; Polizeli, Maria de Lourdes T. M. ^{[1, 3]}

Affiliation

^[1] Univ Sao Paulo, Dept Biochem & Immunol, FMRP, Sao Paulo - Brazil

^[2] Univ British Columbia, Fac Forestry, Dept Wood Sci, Vancouver, BC - Canada

^[3] Univ Sao Paulo, Dept Biol, FFCLRP, Sao Paulo - Brazil

^[4] Univ Estadual Maringa, Dept Biochem, Maringa, Parana - Brazil

• Document type: Journal article
• Source: BIOFUELS BIOPRODUCTS & BIOREFINING-BIOFPR; v. 16, n. 2 NOV 2021.
• Web of Science Citations: 0

Abstract

Perennial grasses, such as Panicum maximum, are important alternatives to dedicated energy crops for bioethanol production. This study investigates whether future climate conditions could influence P. maximum cell-wall structure and hydrolytic performance. To analyze interactions with environmental factors in field conditions, a combined Free-air Temperature and CO2 Controlled Enhancement (Trop-T-FACE) facility was used to investigate the isolated and combined effect of elevated atmospheric CO2 concentration (eC) (600 mu mol.mol(-1)) and elevated temperature (eT) by 2 degrees C more than the ambient temperature, on cell-wall composition, cellulose crystallinity, accessibility, and hydrolysis yields. The elevated temperature treatments (eT and eT + eC) exhibited the most pronounced effects on the P. maximum cell wall. Warming reduced the starch content and the crystallinity index (CI) of cellulose and increased the cellulose content. Fluorescent protein-tagged carbohydrate-binding modules analysis demonstrated that warming improved total cellulose surface exposure/accessibility in eT and eT + eC by 181% and 132%, respectively. Consequently, glucan conversion yields were improved by 7.07% and 5.37%, showing that warming led to lower recalcitrance in P. maximum biomass, which positively affects its use in biorefineries. This work therefore provides important information from an ecological and economic point of view, allowing us to understand the mitigation process applied by this forage grass under future climate conditions. It might assist in selecting tropical forage grasses that are efficiently adapted to climate change, with a positive effect on bioenergy production. (c) 2021 Society of Chemical Industry and John Wiley \& Sons, Ltd (AU)

• FAPESP's process: 08/58075-8 - Miniface climate-change impact experiment to analyze the effects of elevated CO2 and warming on photosynthesis, gene expression, biochemistry, growth, nutrient dynamics and yield of two contrasting tropical forage species
• Grantee: Carlos Alberto Martinez y Huaman
• Support Opportunities: Research Program on Global Climate Change - Thematic Grants
• FAPESP's process: 08/57908-6 - National Institute of Science and Technology of Bioethanol
• Grantee: Marcos Silveira Buckeridge
• Support Opportunities: Program for Research on Bioenergy (BIOEN) - Thematic Grants
• FAPESP's process: 17/23989-9 - Lignocellulosic biomass of Panicum maximum Jacq. forage grass produced in simulated future climate conditions: potential source of fermentable sugars for bioenergy
• Grantee: Emanuelle Neiverth de Freitas
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 19/07141-5 - Formulation of an optimized synergistic enzyme cocktail with accessory enzymes to enhances the hydrolytic performance of Panicum maximum Jacq.
• Grantee: Emanuelle Neiverth de Freitas
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• FAPESP's process: 18/07522-6 - Development of eco-friendly technologies for the hydrolysis of biomass and residues of cellulose pulp and paper industry
• Grantee: Maria de Lourdes Teixeira de Moraes Polizeli
• Support Opportunities: Regular Research Grants