The effects of compound treatment of Aspergillus oryzae and fibrolytic enzyme on in vitro degradation, gas production and fermentative profile of maize silage and sugarcane silage

Author(s)

Souza, J. M. ^[1] ; Souza, J. C. S. M. ^[1] ; Sousa, D. O. ^[2] ; Del Valle, T. A. ^[3] ; Ghizzi, L. G. ^[1] ; Alcantara, A. H. D. ^[4] ; Mesquita, L. G. ^[1] ; Sousa, R. L. M. ^[4] ; Bueno, I. C. S. ^[4] ; Balieiro, J. C. C. ^[1]

Affiliation

^[1] Univ Sao Paulo, Fac Vet Med & Anim Sci, Dept Anim Nutr & Anim Prod, BR-13635900 Pirassununga - Brazil

^[2] Swedish Univ Agr Sci, Dept Anim Environm & Hlth, S-53223 Skara - Sweden

^[3] Fed Univ Pampa, BR-96460000 Itaqui - Brazil

^[4] Univ Sao Paulo, Dept Anim Sci, Facu Anim Sci & Food Engn, BR-13635900 Pirassununga - Brazil

• Document type: Journal article
• Source: JOURNAL OF AGRICULTURAL SCIENCE; v. 159, n. 1-2, p. 147-158, JAN 2021.
• Web of Science Citations: 0

Abstract

The present study was conducted to evaluate the effect of a live culture of Aspergillus oryzae (A; CCT4359) and fibrolytic enzyme (E; Fibrozyme Alltech Inc.) on fibre digestibility by a gas production bioassay and in vitro degradation of maize silage and sugarcane silage. A completely randomized design trial was performed to evaluate: A doses (0, 20, 60 and 100 mg/l), E doses (0, 160, 320 and 480 mg/l) and roughage source (R; maize and sugarcane silage) in a 4 x 4 x 2 factorial arrangement. The inclusion of increasing doses of A and E increased dry matter and neutral detergent fibre in vitro digestibility linearly, but for E this effect occurred only in maize silage. There was a linear increase in the potential for gas production at the highest dose of A only in sugarcane silage, with no effect on lag time (L). Increasing doses of E increased the volume of gases produced linearly, and a trend of linear reduction of L, regardless of the roughage. There was a linear reduction in ammonia-nitrogen concentration in response to increasing doses of A and E, and an increase in acetic acid concentration at the highest dose of A, regardless of roughage. The additives had no synergistic effect on gas production and digestibility, but were efficient in altering the fermentative pattern, demonstrating the potential to increase fibre degradation. (AU)

• FAPESP's process: 17/11537-6 - Supplementation with live cultures of Aspergillus oryzae and fibrolytic enzyme for dairy cattle: Effects on metabolism, kinetics and rumen ecosystem
• Grantee: Johnny Maciel de Souza
• Support Opportunities: Scholarships in Brazil - Doctorate