Artepillin C Reduces Allergic Airway Inflammation by Induction of Monocytic Myeloid-Derived Suppressor Cells

Author(s)

Martins, Nubia Sabrina ^[1] ; de Campos Fraga-Silva, Thais Fernanda ^[2] ; Correa, Giseli Furlan ^[2] ; Boko, Medeton Mahoussi Michael ^[1] ; Ramalho, Leandra Naira Zambelli ^[3] ; Rodrigues, Debora Munhoz ; Hori, Juliana Issa ^[4] ; Costa, Diego Luis ^{[2, 1]} ; Bastos, Jairo Kenupp ^[5] ; Bonato, Vania Luiza Deperon ^{[2, 1]}

Affiliation

^[1] Univ Sao Paulo, Ribeirao Preto Med Sch, Basic & Appl Immunol Program, BR-14049900 Ribeirao Preto, SP - Brazil

^[2] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Biochem & Immunol, BR-14049900 Ribeirao Preto, SP - Brazil

^[3] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Pathol & Legal Med, BR-14049900 Ribeirao Preto, SP - Brazil

^[4] Apis Flora Ind & Comercial Ltda, BR-14020670 Ribeirao Preto, SP - Brazil

^[5] Univ Sao Paulo, Sch Pharmaceut Sci, Dept Pharmaceut Sci, BR-14049900 Ribeirao Preto, SP - Brazil

• Document type: Journal article
• Source: PHARMACEUTICS; v. 13, n. 11 NOV 2021.
• Web of Science Citations: 0

Abstract

Propolis is a natural product produced by bees that is primarily used in complementary and alternative medicine and has anti-inflammatory, antibacterial, antiviral, and antitumoral biological properties. Some studies have reported the beneficial effects of propolis in models of allergic asthma. In a previous study, our group showed that green propolis treatment reduced airway inflammation and mucus secretion in an ovalbumin (OVA)-induced asthma model and resulted in increased regulatory T cells (Treg) and polymorphonuclear myeloid-derived suppressor cells (PMN-MDSC) frequencies in the lungs, two leukocyte populations that have immunosuppressive functions. In this study, we evaluated the anti-inflammatory effects of artepillin C (ArtC), the major compound of green propolis, in the context of allergic airway inflammation. Our results show that ArtC induces in vitro differentiation of Treg cells and monocytic MDSC (M-MDSC). Furthermore, in an OVA-induced asthma model, ArtC treatment reduced pulmonary inflammation, eosinophil influx to the airways, mucus and IL-5 secretion along with increased frequency of M-MDSC, but not Treg cells, in the lungs. Using an adoptive transfer model, we confirmed that the effect of ArtC in the reduction in airway inflammation was dependent on M-MDSC. Altogether, our data show that ArtC exhibits an anti-inflammatory effect and might be an adjuvant therapy for allergic asthma. (AU)

• FAPESP's process: 19/09881-6 - Role of TLR2 and TLR9-dependent signalling in epithelial or dendritic cells in lung inflammation during allergen reexposure and pneumococcal infection
• Grantee: Thais Fernanda de Campos Fraga da Silva
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 19/23446-0 - Evaluation of the role of chemerin in activating the inflammatory response of Mycobacterium tuberculosis infected macrophages
• Grantee: Giseli Furlan Corrêa
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 19/18793-3 - Therapeutic potential of Artepillin C in experimental allergic asthma
• Grantee: Núbia Sabrina Martins
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 17/04138-8 - Attainment of chemical, analytical, biological, pharmacological and technological studies to fill the gaps on the development of Brazilian propolis sector
• Grantee: Jairo Kenupp Bastos
• Support Opportunities: Research Projects - Thematic Grants