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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Modulation of Adhesion Molecules Expression by Different Metalloproteases Isolated from Bothrops Snakes

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Author(s):
Zychar, Bianca C. [1] ; Clissa, Patricia B. [2] ; Carvalho, Eneas [3] ; Alves, Adilson S. [4] ; Baldo, Cristiani [5] ; Faquim-Mauro, Eliana L. [2] ; Goncalves, Luis Roberto C. [1]
Total Authors: 7
Affiliation:
[1] Butantan Inst, Lab Pathophysiol, BR-05503900 Sao Paulo - Brazil
[2] Butantan Inst, Immunopathol Lab, BR-05503900 Sao Paulo - Brazil
[3] Butantan Inst, Bacteriol Lab, BR-05503900 Sao Paulo - Brazil
[4] Univ Sao Paulo, Inst Biomed Sci, Dept Physiol & Biophys, BR-05503900 Sao Paulo - Brazil
[5] Univ Estadual Londrina, Dept Biochem & Biotechnol, BR-86051990 Londrina, Parana - Brazil
Total Affiliations: 5
Document type: Journal article
Source: TOXINS; v. 13, n. 11 NOV 2021.
Web of Science Citations: 0
Abstract

Snake venom metalloproteinases (SVMP) are involved in local inflammatory reactions observed after snakebites. Based on domain composition, they are classified as PI (pro-domain + proteolytic domain), PII (PI + disintegrin-like domains), or PIII (PII + cysteine-rich domains). Here, we studied the role of different SVMPs domains in inducing the expression of adhesion molecules at the microcirculation of the cremaster muscle of mice. We used Jararhagin (Jar)-a PIII SVMP with intense hemorrhagic activity, and Jar-C-a Jar devoid of the catalytic domain, with no hemorrhagic activity, both isolated from B. jararaca venom and BnP-1-a weakly hemorrhagic P1 SVMP from B. neuwiedi venom. Toxins (0.5 mu g) or PBS (100 mu L) were injected into the scrotum of mice, and 2, 4, or 24 h later, the protein and gene expression of CD54 and CD31 in the endothelium, and integrins (CD11a and CD11b), expressed in leukocytes were evaluated. Toxins induced significant increases in CD54, CD11a, and CD11b at the initial time and a time-related increase in CD31 expression. In conclusion, our results suggest that, despite differences in hemorrhagic activities and domain composition of the SVMPs used in this study, they behave similarly to the induction of expression of adhesion molecules that promote leukocyte recruitment. (AU)

FAPESP's process: 08/00108-8 - Role of different domains of Bothrops metaloproteases on the expression of adhesion alteractions leukocyte-endothelium interaction of the microcirculation of murine cremaster: action of proteases inhibitors
Grantee:Bianca Cestari Zychar
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 10/09002-8 - Structural and functional characterization of a protein isolated from Bothrops jararaca snake plasma, related to the mammalian high molecular weight kininogen
Grantee:Luis Roberto de Camargo Gonçalves
Support Opportunities: Regular Research Grants