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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

hotodynamic inactivation of Streptococcus pneumoniae with external illumination at 808 nm through the ex vivo porcine thoracic cag

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Diaz Tovar, Johan Sebastian [1] ; Kassab, Giulia [1] ; Buzza, Hilde Harb [1] ; Bagnato, Vanderlei Salvador [2, 1] ; Kurachi, Cristina [1]
Total Authors: 5
[1] Univ Sao Paulo, Sao Carlos Inst Phys, Phys & Mat Sci Dept, Sao Carlos, SP - Brazil
[2] Texas A&M Univ, College Stn, TX 77843 - USA
Total Affiliations: 2
Document type: Journal article
Source: Journal of Biophotonics; v. 15, n. 2 DEC 2021.
Web of Science Citations: 0

Pneumonia is responsible for high mortality rates around the world, and its major treatment is based on antibiotic treatment. Antimicrobial resistance has been increasing in the last years, resulting in relevant public health concern. A promising alternative for pneumonia is antimicrobial photodynamic therapy. The purpose of this study was to investigate whether 808 nm wavelength is able to be transmitted through the biological tissues of the thoracic wall and be delivered in enough energy inside the cage to activate indocyanine green and promote photodynamic response. A light source panel was developed composed of 200 lasers centered at 808 nm with an irradiance of 77.8 +/- 10.0 mW/cm(2) and tested in an ex vivo thoracic cage model. Monte Carlo simulations were used to understand the photon migration through all the tissues at the thoracic wall. It was observed that tissues responsible for the major absorption of photons are the skin and subcutaneous fat. Experimental measurement of the irradiance was obtained after the light pass-through ex vivo pig thoracic cage, obtaining 3% to 5% of the emitted irradiance. Finally, it was observed that even with 3% of the initial irradiance, a 99.9% reduction of the Streptococcus pneumoniae was successfully achieved after 42.6 minutes of irradiation. (AU)

FAPESP's process: 13/07276-1 - CEPOF - Optics and Photonic Research Center
Grantee:Vanderlei Salvador Bagnato
Support type: Research Grants - Research, Innovation and Dissemination Centers - RIDC
FAPESP's process: 16/14033-6 - Basic studies of the combination of photothermal and photodynamic effects in application of a new modality for the Cancer treatment
Grantee:Hilde Harb Buzzá
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 14/50857-8 - National Institute in Basic Optics and Applied to Life Sciences
Grantee:Vanderlei Salvador Bagnato
Support type: Research Projects - Thematic Grants
FAPESP's process: 18/18188-0 - Photodynamic inactivation of Bacterial Pneumonia with nebulization of the photosensitizer and extracorporeal illumination
Grantee:Giulia Kassab
Support type: Scholarships in Brazil - Doctorate