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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Generation of Primordial Germ Cell-like Cells from iPSCs Derived from Turner Syndrome Patients

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de Souza, Aline Fernanda [1, 2] ; Bressan, Fabiana Fernandes ; Pieri, Naira Caroline Godoy ; Botigelli, Ramon Cesar [3] ; Revay, Tamas [4] ; Haddad, Simone Kashima [5] ; Covas, Dimas Tadeu [5] ; Ramos, Ester Silveira [6] ; King, Willian Allan [2] ; Meirelles, Flavio Vieira [1]
Total Authors: 10
[1] Univ Sao Paulo, Fac Anim Sci & Food Engn, Dept Vet Med, BR-13635000 Pirassununga - Brazil
[2] Univ Guelph, Dept Biomed Sci, Ontario Vet Coll OVC, Guelph, ON N1G 2W1 - Canada
[3] Sao Paulo State Univ UNESP, Inst Biosci IBB, Dept Pharmacol, BR-18618689 Botucatu, SP - Brazil
[4] Univ Calgary, Dept Alberta Childrens Hosp Res Inst ACHRI, Calgary, AB T2N 4N1 - Canada
[5] Univ Sao Paulo, Ribeirao Preto Med Sch, Ctr Cell Based Therapy, Reg Blood Ctr Ribeirao Preto, BR-14051060 Ribeirao Preto - Brazil
[6] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Genet, BR-14049900 Ribeirao Preto - Brazil
Total Affiliations: 6
Document type: Journal article
Source: CELLS; v. 10, n. 11 NOV 2021.
Web of Science Citations: 0

Turner syndrome (TS) is a genetic disorder in females with X Chromosome monosomy associated with highly variable clinical features, including premature primary gonadal failure leading to ovarian dysfunction and infertility. The mechanism of development of primordial germ cells (PGCs) and their connection with ovarian failure in TS is poorly understood. An in vitro model of PGCs from TS would be beneficial for investigating genetic and epigenetic factors that influence germ cell specification. Here we investigated the potential of reprogramming peripheral mononuclear blood cells from TS women (PBMCs-TS) into iPSCs following in vitro differentiation in hPGCLCs. All hiPSCs-TS lines demonstrated pluripotency state and were capable of differentiation into three embryonic layers (ectoderm, endoderm, and mesoderm). The PGCLCs-TS recapitulated the initial germline development period regarding transcripts and protein marks, including the epigenetic profile. Overall, our results highlighted the feasibility of producing in vitro models to help the understanding of the mechanisms associated with germ cell formation in TS. (AU)

FAPESP's process: 19/08346-0 - X-chromosome remodeling in induced pluripotent cells (iPSCs) of Turner syndrome
Grantee:Aline Fernanda de Souza
Support Opportunities: Scholarships abroad - Research Internship - Post-doctor
FAPESP's process: 14/50947-7 - INCT 2014: in Stem Cell and Cell Therapy
Grantee:Dimas Tadeu Covas
Support Opportunities: Research Projects - Thematic Grants
FAPESP's process: 17/12140-2 - X-chromosome status during blood cells reprogramming in human induced pluripotent cells (iPSCs) and generation of in vitro primordial germ cells (iPGCs).
Grantee:Aline Fernanda de Souza
Support Opportunities: Scholarships in Brazil - Post-Doctoral
FAPESP's process: 13/08135-2 - CTC - Center for Cell-Based Therapy
Grantee:Dimas Tadeu Covas
Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC
FAPESP's process: 15/26818-5 - Investigation of cellular and molecular mechanisms on in vitro induced toti- and pluripotency acquisition - a translational approach
Grantee:Fabiana Fernandes Bressan
Support Opportunities: Research Grants - Young Investigators Grants