Capacitive electrical stimulation of a conducting polymeric thin film induces human mesenchymal stem cell osteogenesis

Author(s)

da Silva, Rubens Araujo ^[1] ; Xue, Ruikang ^{[2, 3]} ; de Torresi, Susana Ines Cordoba ^[1] ; Cartmell, Sarah ^{[2, 3]}

Affiliation

^[1] Univ Sao Paulo, Inst Quim, Dept Quim Fundamental, Av Prof Lineu Prestes 748, CP 26077, BR-05508000 Sao Paulo - Brazil

^[2] Univ Manchester, Fac Sci & Engn, Sch Nat Sci, Dept Mat, Royce Hub Bldg, Manchester M13 9PL, Lancs - England

^[3] Univ Manchester, Henry Royce Inst, Royce Hub Bldg, Manchester M13 9PL, Lancs - England

• Document type: Journal article
• Source: BIOINTERPHASES; v. 17, n. 1 JAN 2022.
• Web of Science Citations: 0

Abstract

Electroactive materials based on conductive polymers are promising options for tissue engineering and regenerative medicine applications. In the present work, the conducting copolymers of poly (3,4-ethylenedioxythiophene) and poly (d, l-lactic acid) (PEDOT-co-PDLLA) with PEDOT:PDLLA molar ratios of 1:50, 1:25, and 1:5 were synthesized and compared to the insulating macromonomer of EDOT-PDLLA as an experimental control. Bone marrow-derived human mesenchymal stem cells (hMSC-BM) were cultured on the copolymers and the macromonomer thin films inside a bioreactor that induced a capacitive electrical stimulation (CES) with an electric field of 100 mV/mm for 2 h per day for 21 days. Under CES, the copolymers exhibited good cell viability and promoted the differentiation from hMSC-BM to osteogenic lineages, revealed by higher mineralization mainly when the contents of conducting segments of PEDOT (i.e., copolymer with 1:25 and 1:5 PEDOT:PDLLA ratios) were increased. The results indicate that the intrinsic electrical conductivity of the substrates is an important key point for the effectiveness of the electric field generated by the CES, intending to promote the differentiation effect for bone cells. (AU)

• FAPESP's process: 15/26308-7 - Optimization of the physicochemical properties of nano -structured materials for applications in molecular recognition, catalysis and energy conversion/storage
• Grantee: Roberto Manuel Torresi
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 18/18846-7 - Evaluation of adhesion and proliferation processes of cellular in PEDOT-co-PDLLA thin films using electrical stimulating regimes and mechanical force application
• Grantee: Rubens Araujo da Silva
• Support Opportunities: Scholarships abroad - Research Internship - Post-doctor
• FAPESP's process: 17/08349-3 - Evaluation of the adhesion and proliferation processes of cellular in PEDOT-co-PDLLA thin films supported on Au electrodes functionalized with laminin, integrin and fibronectin
• Grantee: Rubens Araujo da Silva
• Support Opportunities: Scholarships in Brazil - Post-Doctoral