A sugarcane cystatin (CaneCPI-5) alters microcosm biofilm formation and reduces dental caries

Author(s):Show less -

Araujo, Tamara Teodoro ^[1] ; Camiloti, Gabriel Domingues ^[1] ; Valle, Aline Dionizio ^[1] ; Silva, Natara Dias Gomes ^[1] ; Souza, Beatriz Martines ^[1] ; Carvalho, Thamyris de Souza ^[1] ; Camara, Joao Victor Frazao ^[1] ; Shibao, Priscila Yumi Tanaka ^[2] ; Henrique-Silva, Flavio ^[2] ; Cruvinel, Thiago ^[1] ; Magalhaes, Ana Carolina ^[1] ; Buzalaf, Marilia Afonso Rabelo ^[1]

Affiliation

^[1] Univ Sao Paulo, Bauru Sch Dent, Dept Biol Sci, Bauru, SP - Brazil

^[2] Univ Fed Sao Carlos, Dept Genet & Evolut, Lab Mol Biol, Sao Carlos - Brazil

• Document type: Journal article
• Source: BIOFOULING; v. 37, n. 1, p. 109-116, JAN 28 2021.
• Web of Science Citations: 2

Abstract

The antimicrobial and anticaries effects of CaneCPI-5 were evaluated. Ninety bovine enamel samples were treated for 60 s with either phosphate-buffered-saline (PBS), 0.12% chlorhexidine (CHX), 0.05 mg ml(-1) CaneCPI-5, 0.1 mg ml(-1) CaneCPI-5 or 0.5 mg ml(-1) CaneCPI-5. They were incubated with inoculum (human saliva + McBain's saliva) for the first 8 h. From then until the end of the experiment, the enamel was exposed to McBain saliva with sucrose and, once a day, for 5 days, they were treated with the solutions. At the end of the experimental period, resazurin and viable plate count assays were performed. Enamel demineralization was also measured. All concentrations of CaneCPI-5 and CHX significantly reduced the activity of biofilms compared with PBS. For viable plate counts, all treatments similarly reduced the lactobacilli and total streptococci; for the mutans streptococci, 0.05 mg ml(-1) CaneCPI-5 performed better than CHX. All CaneCPI-5 concentrations significantly reduced the integrated mineral loss. This study represents the first step regarding the use of CaneCPI-5 within the concept of acquired enamel pellicle and biofilm engineering to prevent dental caries. (AU)

• FAPESP's process: 19/26070-1 - Modulation of acquired enamel pellicle to control dental mineral loss: unravelling mechanisms to make therapies possible
• Grantee: Marília Afonso Rabelo Buzalaf
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 19/08032-5 - Acquired pellicle engineering for protection against caries and tooth erosion: effect of cystatin, statherin and hemoglobin on the proteomic profile of the acquired pellicle, bacterial biofilm and dental enamel
• Grantee: Tamara Teodoro Araujo
• Support Opportunities: Scholarships in Brazil - Doctorate