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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

ytotoxicity of Reparative Endodontic Cements on Human Periodontal Ligament Stem Cell

Full text
Author(s):
Pedrosa, Marlus da Silva [1] ; Alves, Tomaz [2] ; Rahhal, Juliana Garuba [3] ; Nogueira, Fernando Neves [1] ; Sipert, Carla Renata [2]
Total Authors: 5
Affiliation:
[1] Univ Sao Paulo, Sch Dent, Dept Biomat & Oral Biol, Sao Paulo, SP - Brazil
[2] Univ Sao Paulo, Sch Dent, Dept Stomatol, Sao Paulo, SP - Brazil
[3] Univ Sao Paulo, Sch Dent, Dept Restorat Dent, Sao Paulo, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: PESQUISA BRASILEIRA EM ODONTOPEDIATRIA E CLINICA INTEGRADA; v. 22, 2022.
Web of Science Citations: 0
Abstract

Objective: To compare the cytotoxicity of commercial reparative endodontic cements on human periodontal ligament stem cells (hPDLSCs). Material and Methods: The culture of hPDLSCs was established. Cell density was set at 2 x 104 cells/well in 96-well plates. Extracts of Biodentine, Bio-C Repair, Cimmo HD, MTA Repair HP and White MTA were prepared. Then, the extracts were diluted (pure, 1:4 and 1:16) and inserted into cell-seeded wells for 24, 48, and 72 h to assess cell viability through MTT assay. hPDLSCs incubated with culture medium alone served as a negative control group. Data were analyzed by Two-Way ANOVA and Tukey's test (alpha=0.05). Results: At 24 h, pure extract of MTA Repair HP and Biodentine 1:16 presented higher cell viability compared to control. Lower cell viability was found for pure extract of Cimmo HD, MTA Repair HP 1:4 and 1:16, and White MTA 1:16. At 48 h, pure extract of Bio-C Repair and MTA Repair HP presented higher cell viability compared to control. At 72 h, only the pure extract of MTA Repair HP led to higher cell proliferation compared to control. Conclusion: Biodentine, Bio-C Repair and MTA Repair HP were able to induce hPDLSCs proliferation. Cimmo HD and White MTA were found to be mostly cytotoxic in hPDLSCs. (AU)

FAPESP's process: 17/23158-0 - EFFECT OF PROTEASE ACTIVATED RECEPTOR 1 (PAR1) ACTIVATION ON THE OSTEOGENIC ACTIVITY OF MEMBRANES OF HUMAN PERIODONTAL LIGAMENT MESENCHYMAL STEM CELLS
Grantee:Marinella Holzhausen Caldeira
Support Opportunities: Regular Research Grants
FAPESP's process: 16/13944-5 - Role of inflammatory mediators on the modulation of Cells from Apical Papilla: in vitro study
Grantee:Carla Renata Sipert
Support Opportunities: Regular Research Grants