etallic nanoparticle contamination from environmental atmospheric particulate matter in the last slab of the trophic chain: Nanocrystallography, subcellular localization and toxicity effect

Author(s)

Souza, Iara da C. ^[1] ; Morozesk, Mariana ^[1] ; Siqueira, Priscila ^[1] ; Zini, Enzo ^[2] ; Galter, Iasmini N. ^[2] ; Moraes, Daniel A. de ^[3] ; Matsumoto, Silvia T. ^[2] ; Wunderlin, Daniel A. ^{[4, 5]} ; Elliott, Michael ^{[6, 7]} ; Fernandes, Marisa N. ^[1]

Affiliation

^[1] Univ Fed Sao Carlos DCF UFSCar, Dept Ciencias Fisiol, Ave Washington Luiz, Km 235, BR-13565905 Sao Carlos, SP - Brazil

^[2] Univ Fed Espirito Santo DBV UFES, Dept Ciencias Biol, Ave Fernando Ferrari 514, BR-29075910 Vitoria, ES - Brazil

^[3] Univ Sao Paulo, Inst Quim Sao Carlos, BR-13566590 Sao Carlos, SP - Brazil

^[4] Univ Nacl Cordoba, Fac Ciencias Quim, RA-5000 Cordoba - Argentina

^[5] Univ Nacl Cordoba, CONICET, ICYTAC Inst Ciencia & Tecnol Alimentos Cordoba, RA-5000 Cordoba - Argentina

^[6] Univ Hull, Dept Biol & Marine Sci, Kingston Upon Hull HU6 7RX, N Humberside - England

^[7] Int Estuarine & Coastal Specialists Ltd, Leven HU17 5LQ - Scotland

• Document type: Journal article
• Source: Science of The Total Environment; v. 814, MAR 25 2022.
• Web of Science Citations: 0

Abstract

Atmospheric particulate material (PM) from mining and steel industries comprises several metallic contaminants. PM10 samples collected in a Brazilian region with a recognized influence of the steel and iron pelletizing industries were used to investigate metallic nanoparticle incorporation into human fibroblast cells (MRC-5). MRC-5 cells were exposed to 0 (control, ultrapure water), 2.5, 5, 10, 20 and 40 mu g PM10 mL(-1), for 24 h. Cytotoxic and genotoxic dose-response effects were observed on lysosome and DNA structure, and concentrations high as 20 and 40 mu g PM10 mL(-1) induced elevated cell death. Ultrastructure analyses showed aluminosilicate, iron, and the emerging metallic contaminants titanium, bismuth, and cerium nanoparticles were incorporated into lung cells, in which the nanocrystallography analysis indicated the bismuth as Bi2O3. All internalized metallic nanoparticles were free and unbound in the cytoplasm and nucleus thereby indicating bioavailability and potential interaction to biological processes and cellular structures. Pearson's correlation analysis showed Fe, Ni, Al, Cr, Pb and Hg as the main cytotoxic elements which are associated with the stainless steel production. The presence of internalized nanoparticles in human lung cells exposed to environmental atmospheric matter highlights the need for a greater effort by regulatory agencies to understand their potential damage and hence the need for future regulation, especially of emerging metallic contaminants. (AU)

• FAPESP's process: 19/08491-0 - Atmospheric particulate material and environmental contamination. Impact assessment in the aquatic biota in an integrated ecophysiotoxicological approach
• Grantee: Marisa Narciso Fernandes
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 21/02906-3 - Plasticity, distribution and dynamics of ionocytes and cardiomyocytes: physiological mechanisms and adjustments to environmental contamination in different aquatic environments
• Grantee: Mariana Morozesk
• Support Opportunities: Scholarships in Brazil - Post-Doctoral